A new logic for DNA engineering using recombination in Escherichia coli

被引：940

作者：

Zhang, YM ^{[1
]}

Buchholz, F ^{[1
]}

Muyrers, JPP ^{[1
]}

Stewart, AF ^{[1
]}

机构：

[1] European Mol Biol Lab, Gene Express Program, D-69117 Heidelberg, Germany

来源：

NATURE GENETICS | 1998年 / 20卷 / 02期

关键词：

D O I：

10.1038/2417

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A straightforward way to engineer DNA in E. coli using homologous recombination is described. The homologous recombination reaction uses RecE and RecT and is transferable between E. coli strains. Several target molecules were manipulated, including high copy plasmids, a large episome and the E. coli chromosome. Sequential steps of homologous or site-specific recombination were used to demonstrate a new logic for engineering DNA, unlimited by the disposition of restriction endonuclease cleavage sites or the size of the target DNA.

引用

页码：123 / 128

页数：6

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