Identification of genes differentially expressed during adventitious shoot induction in Pinus pinea cotyledons by subtractive hybridization and quantitative PCR

被引：22

作者：

Alonso, Pablo

Cortizo, Millan

Canton, Francisco R.

Fernandez, Belen

Rodriguez, Ana

Centeno, Maria L.

Canovas, Francisco M.

Ordas, Ricardo J. ^{[1
]}

机构：

[1] Univ Oviedo, Inst Univ Biotecnol Asturias, Dept BOS, Area Fisiol Vegetal, E-33071 Oviedo, Spain

[2] Univ Malaga, Unidad Asociada UMA CSIC, Inst Andaluz Biotecnol, Dept Biol Mol & Bioquim, E-29071 Malaga, Spain

[3] Univ Leon, Fac Ciencias Biol & Ambientales, Dept Biol Vegetal, E-27071 Leon, Spain

来源：

TREE PHYSIOLOGY | 2007年 / 27卷 / 12期

关键词：

caulogenesis; conifers; cytokinins; organogenesis; SSH; stone pine;

D O I：

10.1093/treephys/27.12.1721

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

As part of a study aimed at understanding the physiological and molecular mechanisms involved in adventitious shoot bud formation in pine cotyledons, we conducted a transcriptome analysis to identify early-induced genes during the first phases of adventitious caulogenesis in Pinus pinea L. cotyledons cultured in the presence of benzyladenine. A subtractive cDNA library with more than 700 clones was constructed. Of these clones, 393 were sequenced, analyzed and grouped according to their putative function. Quantitative real-time PCR analysis was performed to confirm the differential expression of 30 candidate genes. Results are contrasted with available data for other species.

引用

页码：1721 / 1730

页数：10