Progesterone-induced reorganisation of NOX-2 components in membrane rafts is critical for sperm functioning in Capra hircus

被引：4

作者：

Shoeb, M. ^{[1
]}

Laloraya, M. ^{[1
]}

Kumar, P. G. ^{[1
]}

机构：

[1] Rajiv Gandhi Ctr Biotechnol, Div Mol Reprod, Thiruvananthapuram 695014, Kerala, India

来源：

ANDROLOGIA | 2010年 / 42卷 / 06期

关键词：

Acrosome reaction; capacitation; NOX; raft; spermatozoa; superoxide; PROTEIN-TYROSINE PHOSPHORYLATION; HUMAN EJACULATED SPERMATOZOA; T-CELL-ACTIVATION; ACROSOME REACTION; LIPID RAFTS; NADPH OXIDASE; MOUSE SPERMATOZOA; HYDROGEN-PEROXIDE; SUPEROXIDE ANION; ENDOTHELIAL-CELLS;

D O I：

10.1111/j.1439-0272.2009.01024.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

P>Spermatozoa produce increasing levels of reactive oxygen species (ROS) during development. We examined the functional organisation of NOX-2 subunits in developing spermatozoa and its involvement in ROS generation. We found NOX-2 components associated predominantly with caveolin-rich microdomains of sperm head and mid piece membranes. Capacitation in vitro enriched NOX-2 components within the caveolin-positive sperm membrane rafts. Progesterone, but not 17 beta-oestradiol, brought about loss of caveolin and extensive reorganisations in NOX-2 distribution on sperm head microdomains and accompanying activation in the levels of superoxide. The sensitivity of superoxide production by spermatozoa to methyl-beta-cyclodextrin confirms that NOX-2 function in spermatozoa is tightly dependent on raft organisation. We conclude that progesterone-induced reorganisation of NOX-2 components in sperm membrane rafts is critical for sperm functioning.

引用

页码：356 / 365

页数：10