Precise and convenient size barcode on microfluidic chip for multiplex biomarker detection

被引:5
|
作者
Tang, Mans [1 ,2 ,3 ]
Chen, Jinyao [1 ]
Lei, Jia [1 ]
Ai, Zhao [1 ,2 ,3 ]
Liu, Feng [1 ,2 ,3 ]
Hong, Shao-Li [1 ,2 ,3 ]
Liu, Kan [1 ,2 ,3 ]
机构
[1] Wuhan Text Univ, Sch Elect & Elect Engn, Wuhan 430200, Peoples R China
[2] Wuhan Text Univ, Hubei Engn & Technol Res Ctr Funct Fibre Fabricat, Wuhan 430200, Peoples R China
[3] Hubei Prov Engn Res Ctr Intelligent Micronano Med, Wuhan 30200, Peoples R China
基金
中国国家自然科学基金;
关键词
HIGH-THROUGHPUT; CELL; TECHNOLOGY; ACID;
D O I
10.1039/d1an01265g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The existing multiplex biomarker detection methods are limited by the high demand for coding material and expensive detection equipment. This paper proposes a convenient and precise coding method based on a wedge-shaped microfluidic chip, which can be further applied in multiplex biomarker detection. The proposed microfluidic chip has a microchannel with continuously varying height, which can naturally separate and code microparticles of different sizes. Our data indicate that this method can be applied to code more than 5 or 7 kinds of microparticles, even when their size discrepancies are smaller than 1 mu m. Based on these, multiplex biomarker detection can be implemented by using microparticles of different sizes, hence each kind of microparticle that coats one kind of antibody represents the species of targets. This method is simple and easy to operate, with no clogging or sophisticated coding design, showing its significant potential in the area of point-of-care tests (POCT).
引用
收藏
页码:5892 / 5897
页数:6
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