Temporal fluctuation in the abundance of alginate-degrading bacteria in the gut of abalone Haliotis gigantea over 1year

被引：15

作者：

Tanaka, Reiji ^{[1
,2
]}

Shibata, Toshiyuki ^{[1
,2
]}

Miyake, Hideo ^{[1
,2
]}

Mori, Tetsushi ^{[2
,3
]}

Tamaru, Yutaka ^{[1
]}

Ueda, Mitsuyoshi ^{[2
,4
]}

Bossier, Peter ^{[5
,6
]}

机构：

[1] Mie Univ, Grad Sch Bioresources, Tsu, Mie, Japan

[2] Japan Sci & Technol Agcy, CREST, Tokyo, Japan

[3] Waseda Univ, Fac Sci & Engn, Tokyo, Japan

[4] Kyoto Univ, Grad Sch Agr, Kyoto, Japan

[5] Univ Ghent, Lab Aquaculture, Ghent, Belgium

[6] Univ Ghent, Artemia Reference Ctr, Ghent, Belgium

来源：

AQUACULTURE RESEARCH | 2016年 / 47卷 / 09期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

alginate; abalone; microbiota; gut; VIBRIO-HALIOTICOLI; DISCUS-HANNAI; SP NOV; INFECTIOUS-DISEASE; GEN; NOV; SP; MICROFLORA; BIODIVERSITY; DEGRADATION; SEAWEED;

D O I：

10.1111/are.12740

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

In this study, we identified and enumerated alginate-degrading bacteria in the gut of abalone over 1-year period. From a total of 360 colonies growing on agar medium enriched with alginate, 251 isolates (70%) had the ability to degrade alginate. In addition, a high number of viable alginate-degrading bacteria were detected throughout the survey period. Alginate-degrading bacteria were more abundant in the cold season relative to thesummer season (10(7) vs. 10(4)CFUg(-1), respectively). Strong positive correlation was also observed between the number of alginate-degrading bacteria and feed intake (R=0.854; P<0.01). The identified alginate-degrading bacteria comprised of 35 species grouped into 11 genera including Algibacter, Formosa, Polarybacter, Tamlana, Tenacibaculum (CFB group), Roseobacter, Ruegeria, Silicibacter (-proteobacteria), Agarivorans, Shewanella and Vibrio (-proteobacteria) respectively. More than 80% of the isolated alginate-degrading bacteria belonged to the genus Vibrio, showing high homology to Vibrio cyclotorophicus, Vibrio splendidus, Vibrio halioticoli and Vibrio neonatus. Based on the results, it was suggested that algal-polysaccharide (alginate) degrading bacteria (mainly Vibrio) commonly exist in the gut of abalone and may play an important role in the degradation and digestion of the host's feed.

引用

页码：2899 / 2908

页数：10