Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis

被引：46
作者：
Kudla, U
Qin, L
Milac, A
Kielak, A
Maissen, C
Overmars, H
Popeijus, H
Roze, E
Petrescu, A
Smant, G
Bakker, J
Helder, J
机构：
[1] Univ Wageningen, Nematol Lab, Grad Sch Expt Plant Sci, NL-6709 PD Wageningen, Netherlands
[2] Acad Romana, Inst Biochem, Bucharest 060031, Romania
来源：
FEBS LETTERS | 2005年 / 579卷 / 11期
关键词：
expansin; plant parasitic nematode; horizontal gene transfer; protein modeling; cell wall modifying proteins;
D O I：
10.1016/j.febslet.2005.03.047
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Southern analysis showed that Gr-EXPB1, a functional expansin from the potato cyst nematode Globodera rostochiensis, is member of a multigene family, and EST data suggest expansins to be present in other plant parasitic nematodes as well. Homology modeling predicted that Gr-EXPB1 domain I (D1) has a flat beta-barrel structure with surface-exposed aromatic rings, whereas the 3D structure of Gr-EXPB1-D2 was remarkably similar to plant expansins. Gr-EXPB1 shows highest sequence similarity to two extracellular proteins from saprophytic soil-inhabiting Actinobacteria, and includes a bacterial type II carbohydrate-binding module. These results support the hypothesis that a number of pathogenicity factors of cyst nematodes is of procaryotic origin and were acquired by horizontal gene transfer. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2451 / 2457
页数：7
相关论文
共 28 条
[1]   Exploiting the past and the future in protein secondary structure prediction [J].
Baldi, P ;
Brunak, S ;
Frasconi, P ;
Soda, G ;
Pollastri, G .
BIOINFORMATICS, 1999, 15 (11) :937-946
[2]   MULTIPLE SEQUENCE ALIGNMENT WITH HIERARCHICAL-CLUSTERING [J].
CORPET, F .
NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10881-10890
[3]   Group I allergens of grass pollen as cell wall-loosening agents [J].
Cosgrove, DJ ;
Bedinger, P ;
Durachko, DM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) :6559-6564
[4]   New genes and new biological roles for expansins [J].
Cosgrove, DJ .
CURRENT OPINION IN PLANT BIOLOGY, 2000, 3 (01) :73-78
[5]   Loosening of plant cell walls by expansins [J].
Cosgrove, DJ .
NATURE, 2000, 407 (6802) :321-326
[6]   USE OF GENOMIC DNA RESTRICTION FRAGMENT LENGTH DIFFERENCES TO IDENTIFY NEMATODE SPECIES [J].
CURRAN, J ;
BAILLIE, DL ;
WEBSTER, JM .
PARASITOLOGY, 1985, 90 (FEB) :137-144
[7]   Secretory granule proteins from the subventral esophageal glands of the potato cyst nematode identified by monoclonal antibodies to a protein fraction from second-stage juveniles [J].
deBoer, JM ;
Smant, G ;
Goverse, A ;
Davis, EL ;
Overmars, HA ;
Pomp, H ;
vanGentPelzer, M ;
Zilverentant, JF ;
Stokkermans, JPWG ;
Hussey, RS ;
Gommers, FJ ;
Bakker, J ;
Schots, A .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1996, 9 (01) :39-46
[8]   C-1-C-X REVISITED - INTRAMOLECULAR SYNERGISM IN A CELLULASE [J].
DIN, N ;
DAMUDE, HG ;
GILKES, NR ;
MILLER, RC ;
WARREN, RAJ ;
KILBURN, DG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11383-11387
[9]  
Harlow E., 1997, ANTIBODIES LAB MANUA
[10]   A polygalacturonase of animal origin isolated from the root-knot nematode Meloidogyne incognita [J].
Jaubert, S ;
Laffaire, JB ;
Abad, P ;
Rosso, MN .
FEBS LETTERS, 2002, 522 (1-3) :109-112
123