Detection of Streptococcus mutans using Padlock Probe Based on Rolling Circle Amplification (RCA)

被引：3
作者：
Moreira, Monica [1 ]
Adamoski, Douglas [3 ]
Sun, Jiufeng [4 ]
Najafzadeh, Mohammad Javad [5 ]
Fidelis do Nascimento, Mariana Machado [2 ]
Gomes, Renata Rodrigues [2 ]
Barbieri, Dicler de St'Anna [2 ]
Glienke, Chirlei [3 ]
Klisiowicz, Debora do Rocio [2 ]
Vicente, Vania Aparecida [1 ,2 ]
机构：
[1] Univ Fed Parana, Programa Pos Grad Engn Bioproc & Biotecnol, BR-80060000 Curitiba, Parana, Brazil
[2] Univ Fed Parana, Programa Pos Grad Microbiol Parasitol & Patol, BR-80060000 Curitiba, Parana, Brazil
[3] Univ Fed Parana, Dept Genet, BR-80060000 Curitiba, Parana, Brazil
[4] China & Univ Farmaceut, Inst Prov Saude Publ Guangdong, Ctr Prov Controle & Prevencao Doencas Guangdong, Escola Ciencias Vida & Tecnol, Nanjing, Jiangsu, Peoples R China
[5] Univ Mashhad Ciencias Med, Dept Parasitol & Micol, Fac Med, Mashhad, Iran
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2015年 / 58卷 / 01期
关键词：
Padlock probe; RCA; Streptococcus mutans; REAL-TIME PCR; RAPID IDENTIFICATION; ORAL STREPTOCOCCI; SEQUENCE-ANALYSIS; DNA; DIFFERENTIATION; ADHERENCE;
D O I：
10.1590/S1516-8913201502762
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutans detection and demonstrated the possibility of a rapid detection and accurate identification of S. mutans infection.
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收藏
页码：54 / 60
页数：7
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