Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

被引：120
作者：
Arduini, Fabiana [1 ,2 ]
Amine, Aziz [3 ]
Moscone, Danila [1 ,2 ]
Palleschi, Giuseppe [1 ,2 ]
机构：
[1] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy
[2] Consorzio Interuniv Biostrutture Biosistemi INBB, I-00136 Rome, Italy
[3] Fac Sci & Tech, Mohammadia, Morocco
来源：
MICROCHIMICA ACTA | 2010年 / 170卷 / 3-4期
关键词：
Biosensors; Inhibition; Insecticides; Cholinesterase; Nerve agents; Aflatoxin; SCREEN-PRINTED ELECTRODES; FIELD-EFFECT TRANSISTORS; NIPPOSTRONGYLUS-BRASILIENSIS ACETYLCHOLINESTERASE; ORGANOPHOSPHATE PESTICIDE DETECTION; SURFACE-PLASMON RESONANCE; SELF-ASSEMBLED MONOLAYER; CHEMICAL WARFARE AGENTS; FLOW-INJECTION ANALYSIS; DURUM-WHEAT SAMPLES; ENZYME-INHIBITION;
D O I：
10.1007/s00604-010-0317-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The present review reports the research carried out during last 9 years on biosensors based on cholinesterase inhibition for nerve agents, organophosphorus and carbammic insecticides, and aflatoxin B-1 detection. Relative applications in environmental and food areas are also reported. Special attention is paid to the optimization of parameters such as enzyme immobilization, substrate concentration, and incubation time in the case of reversible inhibition by aflatoxin B-1 or irreversible inhibition by organophosphorus and carbamic insecticides, and nerve agents in order to optimize and improve the analytical performances of the biosensor. Evaluation of selectivity of the system is also discussed.
引用
收藏
页码：193 / 214
页数：22
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