The apoptosis-inducing protein kinase DRAK2 is inhibited in a calcium-dependent manner by the calcium-binding protein CHP

被引：30

作者：

Kuwahara, H ^{[1
]}

Kamei, J ^{[1
]}

Nakamura, N ^{[1
]}

Matsumoto, M ^{[1
]}

Inoue, H ^{[1
]}

Kanazawa, H ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Dept Biol Sci, Osaka 5600043, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2003年 / 134卷 / 02期

关键词：

apoptosis; apoptosis-inducing protein kinase; calcium-binding protein; calcium signalling;

D O I：

10.1093/jb/mvg137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcineurin homologous protein (CHP) is an EF-hand Ca2+-binding protein capable of interacting with various cellular proteins including Na+/H+ exchangers, kinesin-related proteins, and apoptosis-inducing protein kinase DRAK2. We investigated the role of CHP on the DRAK2 protein kinase in vitro. CHP significantly reduced (similar to85% inhibition) the kinase activity of DRAK2 for both autophosphorylation and phosphorylation of exogenous substrate (myosin light chain). The inhibitory effect of CHP was dependent on the presence of Ca2+, whereas the interaction between CHP and DRAK2 was not Ca2+-dependent. These observations suggest that CHP negatively regulates the apoptosis-inducing protein kinase DRAK2 in a manner that depends on intracellular Ca2+-concentration.

引用

页码：245 / 250

页数：6

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