A gel filtration assay to determine glycogen synthase activity

被引：8

作者：

Niederwanger, A ^{[1
]}

Kranebitter, M ^{[1
]}

Ritsch, A ^{[1
]}

Patsch, JR ^{[1
]}

Pedrini, MT ^{[1
]}

机构：

[1] Med Univ Innsbruck, Clin Dept Internal Med, A-6020 Innsbruck, Austria

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 820卷 / 01期

基金：

奥地利科学基金会;

关键词：

glycogen synthase; glycogen synthase activity; insulin resistance; type-2; diabetes; gel filtration; glycogen; UDP-glucose; glucose-6-phosphate;

D O I：

10.1016/j.jchromb.2005.02.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a gel filtration assay for the determination of glycogen synthase activity in cultured cells or tissue homogenates. Compared to the commonly used filter paper assay, the gel filtration assay resulted in a more than 5-fold reduction of background levels leading to an at least - twofold increase in precision. These benefits allow the gel filtration method to detect differences of +/- 5% in enzyme activity out of 300 mu g total cell protein. In addition to high precision and sensitivity, the method's additional salient advantages include lesser expenditure of time and labour and reduced exposure time of the personnel to radioactivity. (c) 2005 Published by Elsevier B.V.

引用

页码：143 / 145

页数：3

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