Influence of earlywood-latewood size and isotope differences on long-term tree-ring δ13C trends

Inter-annual delta C-13 values of alpha-cellulose in two Chinese pine trees (Pinus tabulaeformis Carr.) exhibit a long-term decline of more than 2 parts per thousand for the period of 1896 to 1990 AD. This decline is much larger than the decrease in atmospheric delta C-13 caused by industrialization since 1850 AD. Here we present evidence that long-term trends of C-13/C-12 in tree rings also reflect ecophysiological effects superimposed on the anthropogenic trend. These effects are associated with the decreasing/increasing productivity of earlywood or latewood associated with the changing growth history of the tree. Theoretical calculations show that long-term decreases in the C-13/C-12 ratios in tree rings may be enhanced or reduced by these ecophysiological effects, depending on the pattern of seasonal differences in tree-ring delta C-13 values. Based on seasonal delta C-13 analyses, the influence of these effects on delta C-13 values for one of these trees (HL02) has been removed. The corrected data are in agreement with the observed size and timing of the delta C-13 decline in atmospheric CO2. This study suggests that long-term tree-ring delta C-13 data used to infer the anthropogenic impact on atmospheric CO2 carbon isotope compositions may be improved by correcting for possible ecophysiological effects using measurement of latewood and earlywood ring widths and seasonally resolved isotopic analysis. Removal of growth effects may also benefit the assessment of long-term changes in the water use efficiency in response to increasing atmospheric CO2 concentrations. (c) 2004 Elsevier B.V. All rights reserved.