Molecular typing of canine parvovirus with PCR assay based on genetic markers of the antigenic variants

被引：0
作者：
Singh, Deepti [1 ]
Verma, Amit Kumar [1 ]
Kumar, Amit [2 ]
Srivastava, Mukesh Kumar [3 ]
机构：
[1] Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu C, Dept Vet Epidemiol & Prevent Med, Mathura 281001, Uttar Pradesh, India
[2] Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu C, Dept Vet Microbiol & Immunol, Mathura 281001, Uttar Pradesh, India
[3] Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu C, Dept Vet Clin Med, Coll Biotechnol, Mathura 281001, Uttar Pradesh, India
来源：
INDIAN JOURNAL OF ANIMAL SCIENCES | 2015年 / 85卷 / 01期
关键词：
Canine parvovirus; Dog; India; Polymerase chain reaction; POLYMERASE-CHAIN-REACTION; VP2; GENE; PHYLOGENETIC ANALYSIS; STRAINS; EVOLUTION; DOGS; SEQUENCES; ISOLATE; BRAZIL; INDIA;
D O I：
暂无
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The canine parvovirus (CPV) is prevalent in India but only scanty literature is available on molecular differentiation of its strain using polymerase chain reaction. In the present study, 100 faecal samples from dogs suspected for canine parvo virus infection (gastroenteritis) were collected and processed for isolation of viral genomic DNA. Molecular test like PCR assay using 3 specific pairs of primers (pCPV-RT, pCPV-2ab and pCPV-2b) was conducted for differential diagnosis of the virus type. Out of 100 faecal samples, 63 were positive for presence of CPV, of which 54 were CPV-2b and 9 were CPV-2a, indicating the predominance of CPV-2b virus type.
引用
收藏
页码：12 / 15
页数：4
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