Properties of methoxy(polyethylene glycol)-lipase from Candida rugosa in organic solvents

被引：0

作者：

Zacchigna, M ^{[1
]}

Di Luca, G ^{[1
]}

Lassiani, L ^{[1
]}

Varnavas, A ^{[1
]}

Boccù, E ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy

来源：

FARMACO | 1998年 / 53卷 / 12期

关键词：

lipase; methoxy(polyethylene glycol); protein modification; esterification; enantioselectivity; enzymes in organic solvents;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Lipase from candida rugosa was modified with methoxy(polyethylene glycol)-p-nitrophenyl carbonate (NPC-mPEG). A total of 34 out of 35 lysine chains were substituted, and the modified lipase retained 98% of the hydrolytic activity of the non-modified enzyme, mPEG-lipase is perfectly soluble in organic solvent and efficiently catalyzes the esterification of lauric acid, with nearly 100% of yield over 6 h in toluene. Moreover, mPEG-lipase exhibits a preference for the S-isomer over the R-isomer in the stereoselective resolution of racemic naproxen. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：758 / 763

页数：6

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