Enzymatic production of xylooligosaccharides from alkali solubilized xylan of natural grass (Sehima nervosum)

被引:95
作者
Samanta, A. K. [1 ]
Jayapal, Natasha [1 ]
Kolte, A. P. [1 ]
Senani, S. [1 ]
Sridhar, Manpal [1 ]
Suresh, K. P. [1 ]
Sampath, K. T. [1 ]
机构
[1] Natl Inst Anim Nutr & Physiol, Bangalore 560030, Karnataka, India
关键词
Sehima nervosum grass; Xylan; Xylooligosaccharide; Lignocellulosic biomass; RSM; GRADED ETHANOL PRECIPITATION; STRUCTURAL-CHARACTERIZATION; POLYSACCHARIDES; HEMICELLULOSE; PEROXIDE; FIBER;
D O I
10.1016/j.biortech.2012.02.036
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In this study, a process for producing XOS from Sehima nervosum grass was developed. The grass contains 28.1% hemicellulose. NaOH and steam application yielded 98% of original xylan in contrast to 85% by KOH application. Hydrolysis of xylan with commercial xylanase caused breakdown into XOS comprising of xylobiose, xylotriose along with xylose. Response surface model (RSM) revealed highest xylobiose yield (11 g/100 g xylan) at pH 5.03, temperature 45.19 degrees C, reaction time 10.11 h with enzyme dose 17.41 U. Similarly for maximizing xylotriose yield, ideal hydrolysis conditions were pH 5.11, temperature 40.33 degrees C, reaction time 16.55 h with enzyme dose 13.20 U. A two step process encompassing xylan fractionation and enzymatic hydrolysis enabled XOS production from the S. nervosum grass. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:199 / 205
页数:7
相关论文
共 34 条
[1]   Xylooligosaccharides (XOS) as an Emerging Prebiotic: Microbial Synthesis, Utilization, Structural Characterization, Bioactive Properties, and Applications [J].
Aachary, Ayyappan Appukuttan ;
Prapulla, Siddalingaiya Gurudutt .
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2011, 10 (01) :2-16
[2]   Enzymatic production of xylooligosaccharides from cotton stalks [J].
Akpinar, Ozlem ;
Ak, Ozlem ;
Kavas, Aysegul ;
Bakir, Ufuk ;
Yilmaz, Levent .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (14) :5544-5551
[3]   Enzymatic production of Xylooligosaccharide from selected agricultural wastes [J].
Akpinar, Ozlem ;
Erdogan, Kader ;
Bostanci, Seyda .
FOOD AND BIOPRODUCTS PROCESSING, 2009, 87 (C2) :145-151
[4]   Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review [J].
Alvira, P. ;
Tomas-Pejo, E. ;
Ballesteros, M. ;
Negro, M. J. .
BIORESOURCE TECHNOLOGY, 2010, 101 (13) :4851-4861
[5]   Structural characterization and cytotoxic properties of a 4-O-methylglucuronoxylan from Castanea sativa.: 2.: Evidence of a structure-activity relationship [J].
Barbat, Aline ;
Gloaguen, Vincent ;
Moine, Charlotte ;
Sainte-Catherine, Odile ;
Kraemer, Michel ;
Rogniaux, Helene ;
Ropartz, David ;
Krausz, Pierre .
JOURNAL OF NATURAL PRODUCTS, 2008, 71 (08) :1404-1409
[6]   Xylooligosaccharides Production from Alkali-Pretreated Sugarcane Bagasse Using Xylanases from Thermoascus aurantiacus [J].
Brienzo, Michel ;
Carvalho, Walter ;
Milagres, Adriane M. F. .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2010, 162 (04) :1195-1205
[7]   Structural elucidation of hemicelluloses from Vetiver grass [J].
Chaikumpollert, O ;
Methacanon, P ;
Suchiva, K .
CARBOHYDRATE POLYMERS, 2004, 57 (02) :191-196
[8]   Acidic heteroxylans from medicinal plants and their anti-ulcer activity [J].
Cipriani, Thales R. ;
Mellinger, Caroline G. ;
de Souza, Lauro M. ;
Baggio, Cristiane H. ;
Freitas, Cristina S. ;
Marques, Maria C. A. ;
Gorin, Philip A. J. ;
Sassaki, Guilherme L. ;
Iacomini, Marcello .
CARBOHYDRATE POLYMERS, 2008, 74 (02) :274-278
[9]   Isolation of hemicellulose from corn fiber by alkaline hydrogen peroxide extraction [J].
Doner, LW ;
Hicks, KB .
CEREAL CHEMISTRY, 1997, 74 (02) :176-181
[10]   Immunomodulatory activity of acidic xylans in relation to their structural and molecular properties [J].
Ebringerová, A ;
Kardosová, A ;
Hromádková, Z ;
Malovíková, A ;
Hríbalová, V .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2002, 30 (01) :1-6