Biodegradable amphiphilic copolymers based on poly(ε-caprolactone)-graft chondroitin sulfate as drug carriers

被引:31
作者
Chen, Ai-Ling [1 ]
Ni, Hsiao-Chen [1 ]
Wang, Li-Fang [1 ]
Chen, Jenn-Shing [2 ]
机构
[1] Kaohsiung Med Univ, Fac Med & Appl Chem, Sch Life Sci, Kaohsiung 80708, Taiwan
[2] Natl Univ Kaohsiung, Dept Appl Chem, Kaohsiung 80811, Taiwan
基金
美国国家科学基金会;
关键词
D O I
10.1021/bm800485x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The goal of this study was to develop a new type of core-shell micelles based on biocompatible and biodegradable amphiphilic copolymers, named PCL-CS, using chondroitin sulfate (CS) as a hydrophilic segment and poly(epsilon-caprolactone) (PCL) as a hydrophobic segment. The copolymers, prepared from the various compositions between CS and PCL, were characterized by Fourier transform infrared spectrometer, proton nuclear magnetic resonance spectrometer, and differential scanning calorimeter. The PCL-CS copolymers could be assembled into micelles using a simple emulsion. With the fluorescent probe technique, the critical micelle concentrations were obtained in the range of 1.26 x 10(-3) -8.86 x 10(-3) mg/mL. The spherical images of micelles were visualized in the presence of polyvinyl alcohol (PVA) with the use of the transmission electron microscope. The particle sizes of micelles were all smaller than 300 nm, neither aggregate nor change in hydrodynamic sizes after 15 days staying in solutions containing salts or PVA by dynamic light scattering. The intracellular uptake of KB cells incubated with PCL-CS micelles was evidenced by confocal laser scanning microscope upon loading fluorescein isothiocyanate labeled bovine serum albumin as a probe.
引用
收藏
页码:2447 / 2457
页数:11
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