Three-dimensional encapsulation of adult mouse cardiomyocytes in hydrogels with tunable stiffness

被引：29

作者：

Crocini, Claudia ^{[1
,2
]}

Walker, Cierra J. ^{[2
,3
]}

Anseth, Kristi S. ^{[1
,2
,4
]}

Leinwand, Leslie A. ^{[1
,2
]}

机构：

[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

[2] Univ Colorado, BioFrontiers Inst, Boulder, CO 80303 USA

[3] Univ Colorado, Mat Sci & Engn Program, Boulder, CO 80303 USA

[4] Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80303 USA

来源：

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | 2020年 / 154卷

基金：

美国国家卫生研究院;

关键词：

AXIAL TUBULAR SYSTEM; VENTRICULAR MYOCYTES; HEART-FAILURE; RAT; CONTRACTILITY; TRANSDUCTION; MATURATION; INTEGRINS; SARCOMERE; FIBROSIS;

D O I：

10.1016/j.pbiomolbio.2019.04.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous diseases, including those of the heart, are characterized by increased stiffness due to excessive deposition of extracellular matrix proteins. Cardiomyocytes continuously adapt their morphology and function to the mechanical changes of their microenvironment. Because traditional cell culture is conducted on substrates that are many orders of magnitude stiffer than any environment encountered by a cardiomyocyte in health or disease, alternate culture systems are necessary to model these processes in vitro. Here, we employ photo-clickable thiol-ene poly(ethylene glycol) (PEG) hydrogels for three-dimensional cell culture of adult mouse cardiomyocytes. PEG hydrogels serve as versatile biocompatible scaffolds, whose stiffness can be precisely tuned to mimic physiological and pathological micro-environments. Compared to traditional culture, adult cardiomyocytes encapsulated in PEG hydrogels exhibited longer survival and preserved sarcomeric and T-tubular architecture. Culture in PEG hydrogels of varying stiffnesses regulated the subcellular localization of the mechanosensitive transcription factor, YAP, in adult cardiomyocytes, indicating PEG hydrogels offer a versatile platform to study the role of mechanical cues in cardiomyocyte biology. (C) 2019 Elsevier Ltd. All rights reserved.

引用

页码：71 / 79

页数：9

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