A Monoclonal Antibody Against the Oncogenic Mucin 1 Cytoplasmic Domain

被引：18

作者：

Panchamoorthy, Govind ^{[2
]}

Rehan, Hala ^{[2
]}

Kharbanda, Akriti ^{[2
]}

Ahmad, Rehan

Kufe, Donald ^{[1
]}

机构：

[1] Harvard Univ, Dana Farber Canc Inst, Dept Med Oncol, Sch Med, Boston, MA 02215 USA

[2] Genus Oncol, Boston, MA USA

来源：

HYBRIDOMA | 2011年 / 30卷 / 06期

关键词：

MUC1-C ONCOPROTEIN; BREAST; REACTIVITY; ACTIVATION; DF3;

D O I：

10.1089/hyb.2011.0070

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mucin 1 (MUC1) is a heterodimeric protein that is aberrantly overexpressed in diverse human carcinomas and certain hematologic malignancies. The transmembrane MUC1-C subunit confers tumorigenicity and is a target for anti-cancer drug development. In this regard, the MUC1-C cytoplasmic domain interacts with multiple effectors that have been linked to transformation. Here we report on the generation of a mouse monoclonal antibody (MAb) against the human MUC1-C cytoplasmic domain (MUC1-CD). This IgG1 MAb, designated anti-MUC1-CD, reacts with the NYGQLDIFP epitope. We show that anti-MUC1-CD is useful in immunoblotting and immunoprecipitation experiments. In addition, anti-MUC1-CD can be used to detect expression of the MUC1-C subunit in formalin-fixed, paraffin-embedded tissues. The MUC1-C inhibitor has entered Phase I evaluation for patients with refractory solid tumors. The present results indicate that the anti-MUC1-CD antibody could be useful as a biomarker to identify patients with tumors that may be responsive to MUC1-C inhibitors.

引用

页码：531 / 535

页数：5

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