A Responsive Hidden Toehold To Enable Controllable DNA Strand Displacement Reactions

被引：85

作者：

Xing, Yongzheng ^{[1
,2
]}

Yang, Zhongqiang ^{[1
]}

Liu, Dongsheng ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Organ Optoelect & Mol Engn, Minist Educ, Beijing 100084, Peoples R China

[2] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 50期

基金：

中国国家自然科学基金;

关键词：

aptamers; DNA structures; hidden toehold; nanotechnology; strand displacement reaction; APTAMERS; HYBRIDIZATION; NANOMACHINES; ADENOSINE; EVOLUTION; SENSORS; LIGANDS; ENZYME;

D O I：

10.1002/anie.201105923

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the loop: A hidden toehold in a metastable DNA bulge-loop structure allows regulation of DNA strand displacement reactions by the addition of ATP, which can bind to an aptamer located partly on the loop sequence (see picture, blue and green in A), thereby exposing the hidden toehold (green and red in Q). By introducing other aptamer sequences, this regulation mechanism could be easily extended to a variety of other ligands. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：11934 / 11936

页数：3

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