Novel preloaded resins for solid-phase biotinylation of carboxylic acids

被引：5

作者：

Cankarova, Nadezda ^{[1
]}

Funk, Petr ^{[1
]}

Hlavac, Jan ^{[1
]}

Soural, Miroslav ^{[1
]}

机构：

[1] Palacky Univ, Fac Sci, Inst Mol & Translat Med, Dept Organ Chem, Olomouc 77146, Czech Republic

来源：

TETRAHEDRON LETTERS | 2011年 / 52卷 / 44期

关键词：

Solid-phase synthesis; Biotine; PEG linker; Acylation; Affinity chromatography; RECOMBINANT ANTIBODY MICROARRAYS; AFFINITY-CHROMATOGRAPHY; PEPTIDES; PROTEINS; DESIGN;

D O I：

10.1016/j.tetlet.2011.08.115

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Use of solid-phase synthesis for the derivatization of carboxylic acids with biotinylated spacers consisting of ethylenoxy units is described. An aminomethylated resin provided with an acid-labile aldehyde linker is used as the polymer support and three different systems with a reactive amino group are introduced. Acylation of each system was tested with a set of model carboxylic acids and afforded crude products of excellent purity. The preloaded resins are similar to the Biotin-PEG-NovaTagTM resin but offer several advantages including simple elongation of the spacer arm. The protocols described represent a very efficient way of modifying compounds to obtain ligands for affinity chromatography studies. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：5782 / 5788

页数：7

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