Isolation and molecular characterization of a naturally occurring non-structural protein 5 (NSP5) gene reassortant of group a rotavirus of serotype G2P[4] with a long RNA pattern

We report here two unusual strains of group A rotavirus, AU85 and AU102, isolated from children with diarrhea. These strains showed an unusual combination of serotype G2 and a long RNA pattern. RNA-RNA hybridization assays showed that these strains are reassortants in which a single genome segment 11 (the NSP5 gene) was derived from a Wa genogroup strain, while other 10 genome segments from a DS-1 genogroup strain. Phylogenetic analysis showed that the NSP5 gene of strain AU85 did not form cluster with Wa strain, while it belonged to the cluster of YM and other porcine strains. Phylogenetic analysis also showed that NSP5 and VP7 genes of AU85 were derived from the rotavirus circulating in the area. Both co-electrophoresis and RNA-RNA hybridization showed that AU85 and AU102 are identical strains. Moreover, the nucleotide sequence comparison between these two strains revealed that they had 100% identical NSP4, NSP5, and VP7 genes. These results suggest that AU85 was a reassortant formed relatively recently between rotaviruses belonging to the Wa and the DS-1 genogroup.