Candida rugosa lipase immobilization on hydrophilic charged gold nanoparticles as promising biocatalysts: Activity and stability investigations

被引：50

作者：

Venditti, Iole ^{[1
]}

Palocci, Cleofe ^{[1
]}

Chronopoulou, Laura ^{[1
]}

Fratoddi, Ilaria ^{[1
,2
]}

Fontana, Laura ^{[1
]}

Diociaiuti, Marco ^{[3
]}

Russo, Maria Vittoria ^{[1
,4
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Ctr Nanotechnol Engn CNIS, I-00185 Rome, Italy

[3] Ist Super Sanita, Technol & Hlth Dept, I-00161 Rome, Italy

[4] Univ Roma La Sapienza, Ctr Protect Environm & Cultural Heritages CIABC, I-00185 Rome, Italy

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 131卷

关键词：

Gold nanoparticles; Candida rugosa lipase; Lipase immobilization; Biocatalysis; PH-INDUCED AGGREGATION; SILVER NANOPARTICLES; KINETIC RESOLUTION; TRANSESTERIFICATION; NANOSPHERES; BIODIESEL; DELIVERY; ENZYMES; HYBRIDS;

D O I：

10.1016/j.colsurfb.2015.04.046

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work, a simple and versatile methodology to obtain two different bioconjugated systems has been developed by the immobilization of Candida rugosa lipase (CRL) on hydrophilic gold nanoparticles functionalized with 2-diethylaminoethanethiol hydrochloride (DEA) or with sodium 3-mercapto-1-propanesulfonate (3MPS), namely Au-DEA@CRL and Au-3MPS@CRL. Both spectroscopic and morphological properties of metal nanoparticles have been deeply investigated. The enzyme loading and lipolytic activity of AuNPs@CRL bio conjugates have been studied with respect to different surface functionalization and compared with the free enzyme. Some physical and chemical parameters had a strong effect on enzyme activity and stability, that were improved in the case of the Au-DEA@CRL bioconjugate, which showed a remarkable biocatalytic performance (95% of residual lipolytic activity compared with free CRL) and stability in experimental conditions concerning pH (range 5-8) and temperature (range 20-60 degrees C), as often required for the industrial scale up of catalytic systems. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：93 / 101

页数：9