Real-time binding kinetic analyses of the interaction of the dietary stain orange II with dentin matrix

被引:2
作者
Alraies, Amr [1 ]
Cole, David K. [2 ,3 ]
Rees, Jeremy S. [4 ]
Glasse, Carl [5 ]
Young, Nigel [5 ]
Waddington, Rachel J. [1 ]
Sloan, Alastair J. [1 ]
机构
[1] Cardiff Univ, Coll Biomed & Life Sci, Sch Dent, Mineralised Tissue Grp,Oral & Biomed Sci, Heath Pk, Cardiff CF14 4XY, S Glam, Wales
[2] Cardiff Univ, Sch Med, Div Infect & Immun, Heath Pk, Cardiff CF14 4XN, S Glam, Wales
[3] Cardiff Univ, Sch Med, Syst Immun Res Inst, Heath Pk, Cardiff CF14 4XN, S Glam, Wales
[4] Cardiff Univ, Coll Biomed & Life Sci, Sch Dent, Heath Pk, Cardiff, S Glam, Wales
[5] Philips Res Labs, 101 Cambridge Sci Pk,Milton Rd, Cambridge CB4 0FY, England
关键词
Orange II; Dentin; Collagen type I; Hydrogen peroxide; Tooth staining; Tooth whitening; HYDROGEN-PEROXIDE; PULP CHAMBER; PENETRATION; TEETH; COMPONENTS; CHEMISTRY; EFFICACY; OFFICE;
D O I
10.1016/j.jdent.2018.06.001
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: Dietary stains can be adsorbed into the dentin of teeth. Using Orange II as a model dietary stain, this study investigated the strength of its interaction with the mineral and protein components of dentin matrix and how hydrogen peroxide (H2O2) treatment influences this interaction. Methods: Dentin slices were prepared from human teeth and were either deproteinized (5.6% sodium hypochlorite, 12 days), demineralised (0.5 M EDTA, 3 days) or left as intact control samples. Samples were stained with Orange II for 1-168 h, during which staining intensity was quantified by image analysis. Similarly, uptake of stain by deproteinized / demineralized samples treated with 10 or 30% H2O2 was investigated. Using surface plasmon resonance technology, real-time binding kinetics were determined assessing the interaction of Orange II with the dentin matrix protein constituents, collagen type I, biglycan, decorin, dentin sialoprotein and osteopontin. Results: Deproteinization of dentin matrix reduced the uptake of the Orange II compared to the intact control. Conversely, demineralization of dentin samples increased the uptake of the dye. Treatment of samples for 48 h with H2O2 reduced subsequent uptake of the Orange II. Real-time kinetic analysis indicated moderate strength of binding for Orange II with collagen type I, weak binding with decorin and biglycan and negligible binding with dentine sialoprotein and osteopontin. Conclusion: These results indicate a predominant role for collagen type I, which accounts for 90% of the organic protein matrix of teeth, for attracting dietary stains. Binding analyses indicate that the interaction is highly dissociable, and further binding is reduced following H2O2 treatment. Clinical significance: This study provides new information regarding adsorption of dietary stains into tooth dentin, suggesting that they are attracted and moderately bound to the collagen type I matrix. This study also contributes valuable information for discussion for considering the effect of H2O2 on bleaching teeth and its influence on subsequent uptake of dietary stains following whitening treatments.
引用
收藏
页码:80 / 88
页数:9
相关论文
共 33 条
  • [1] Penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique
    Afonso Camargo, Samira Esteves
    Valera, Marcia Carneiro
    Ribeiro Camargo, Carlos Henrique
    Gasparoto Mancini, Maria Nadir
    Menezes, Marcia Maciel
    [J]. JOURNAL OF ENDODONTICS, 2007, 33 (09) : 1074 - 1077
  • [2] [Anonymous], DENT UPDATE
  • [3] [Anonymous], DENT UPDATE
  • [4] [Anonymous], 1997, J AM DENT ASSOC, DOI DOI 10.14219/JADA.ARCHIVE.1997.0428
  • [5] Auschill TM, 2005, OPER DENT, V30, P156
  • [6] The effect of enamel proteins on erosion
    Baumann, T.
    Carvalho, T. S.
    Lussi, A.
    [J]. SCIENTIFIC REPORTS, 2015, 5
  • [7] Multiple interactions between polyphenols and a salivary proline-rich protein repeat result in complexation and precipitation
    Baxter, NJ
    Lilley, TH
    Haslam, E
    Williamson, MP
    [J]. BIOCHEMISTRY, 1997, 36 (18) : 5566 - 5577
  • [8] In vitro penetration of bleaching agents into the pulp chamber
    Benetti, AR
    Valera, MC
    Mancini, MNG
    Miranda, CB
    Balducci, I
    [J]. INTERNATIONAL ENDODONTIC JOURNAL, 2004, 37 (02) : 120 - 124
  • [10] BOWES JH, 1950, BIOCHEM J, V46, P1