Direct writing of biocatalytic materials based on pens filled with high-tech enzymatic inks: "Do-it-Yourself"

被引：16

作者：

Hassanpour, Soodabeh ^{[1
]}

Saadati, Arezoo ^{[1
]}

Hasanzadeh, Mohammad ^{[2
]}

Shadjou, Nasrin ^{[3
,4
]}

Mirzaie, Arezo ^{[1
]}

Jouyban, Abolghasem ^{[1
]}

机构：

[1] Tabriz Univ Med Sci, Pharmaceut Anal Res Ctr, Tabriz, Iran

[2] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz 51664, Iran

[3] Urmia Univ, Nanotechnol Res Ctr, Dept Nanochem, Orumiyeh 57154, Iran

[4] Urmia Univ, Fac Sci, Dept Nano Technol, Orumiyeh 57154, Iran

来源：

MICROCHEMICAL JOURNAL | 2019年 / 145卷

关键词：

Biocatalytic materials; Nano-ink; Advanced nanomaterial; Biocompatible agents; Enzymatic inks; PHENYLALANINE-DEHYDROGENASE; PROLINE DEHYDROGENASE; IMMOBILIZATION; NANOMATERIAL; PESTICIDES; METABOLISM;

D O I：

10.1016/j.microc.2018.10.050

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme conductive ink was synthesized and applied for the sensitive detection of L-proline in human bio-fluids. The nano-ink is a mixture of materials graphite (Gr), intermediates Methylene blue (MB), binding agents (Polyethylene glycol and chitosan), and Proline dehydrogenase (PRODH). The morphology of enzymatic ink composition was evaluated by using high-resolution field emission scanning electron microscope (FE-SEM). The results showed that the particle size of the proposed ink was below 100 nm. For electrochemical testing, the ink was fixed on the surface of copper electrode through utilization drop-casting technique via strong bonding between PEG and surface. The modified electrode was used as an electrochemical biosensor for the determination of L-proline in the alkaline solution. In optimal conditions, the concentration range and low limit of quantification (LLOQ) were 56 mu M-1 mM and 56 mu M, respectively.

引用

页码：266 / 272

页数：7

共 25 条

[1] Biocompatible Enzymatic Roller Pens for Direct Writing of Biocatalytic Materials: "Do-it-Yourself" Electrochemical Biosensors [J].