Sensitive Detection of Spores Using Volume-Amplified Magnetic Nanobeads

被引：28
作者：
de la Torre, Teresa Zardan Gomez [1 ]
Ke, Rongqin [2 ]
Mezger, Anja [2 ]
Svedlindh, Peter [3 ]
Stromme, Maria [1 ]
Nilsson, Mats [2 ]
机构：
[1] Uppsala Univ, Angstrom Lab, Div Nanotechnol & Funct Mat, Dept Engn Sci, S-75121 Uppsala, Sweden
[2] Uppsala Univ, Rudbeck Lab, Dept Immunol Genet & Pathol Sci Life Lab, S-75185 Uppsala, Sweden
[3] Uppsala Univ, Angstrom Lab, Div Solid State Phys, Dept Engn Sci, S-75121 Uppsala, Sweden
来源：
SMALL | 2012年 / 8卷 / 14期
基金：
瑞典研究理事会;
关键词：
biosensors; immunoassays; magnetic particles; spores; nanobeads; PROXIMITY LIGATION ASSAYS; DNA-COILS; NANOPARTICLES; BIOSENSORS; ATTACHMENT;
D O I：
10.1002/smll.201102632
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A magnetic-nanobead-based, substrate-free method for the sensitive detection of spores in an immunoassay format is presented. The method is shown to detect Bacillus globigii spores, the non-pathogenic simulant of Bacillus anthracis, with a limit-of-detection of 50 spores with a reaction time of 135 min. The study shows the versatility of magnetic nanobeads for detection of biological molecules other than DNA. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：2174 / 2177
页数：4
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