A MoS2-based system for efficient immobilization of hemoglobin and biosensing applications

被引：65

作者：

Chao, Jie ^{[1
]}

Zou, Min ^{[1
]}

Zhang, Chi ^{[1
]}

Sun, Haofan ^{[1
]}

Pan, Dun ^{[2
]}

Pei, Hao ^{[2
]}

Su, Shao ^{[1
]}

Yuwen, Lihui ^{[1
]}

Fan, Chunhai ^{[1
,2
]}

Wang, Lianhui ^{[1
]}

机构：

[1] Nanjing Univ Posts & Telecommun, Natl Synergist Innovat Ctr Adv Mat SICAM, IAM, KLOEID, Nanjing 210023, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China

来源：

NANOTECHNOLOGY | 2015年 / 26卷 / 27期

基金：

中国国家自然科学基金;

关键词：

AuNPs@MoS2 nanocomposite; electrochemical biosensor; hydrogen peroxide; nitric oxide; DIRECT ELECTRON-TRANSFER; HYDROGEN-PEROXIDE; DIRECT ELECTROCHEMISTRY; MOS2; NANOSHEETS; HORSERADISH-PEROXIDASE; CATALYTIC-ACTIVITY; AU NANOPARTICLES; ELECTROCATALYSIS; GRAPHENE; OXYGEN;

D O I：

10.1088/0957-4484/26/27/274005

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A novel hydrogen peroxide (H2O2) and nitric oxide (NO) biosensor was fabricated by immobilizing hemoglobin (Hb) on a gold nanoparticle-decorated MoS2 nanosheet (AuNPs@MoS2) nanocomposite film modified glass carbon electrode. The AuNPs@MoS2 nanocomposite not only made the immobilized Hb keep its native biological activity but also facilitated the electron transfer between electrode and the electroactive center of Hb due to its excellent conductivity and biocompatibility. The direct electrochemistry and bioelectrocatalytic activity of Hb were investigated by cyclic voltammetry (CV). The modified electrode showed good electrocatalytic ability toward the reduction of H2O2 and NO. Under optimal conditions, the current response was linear with the concentration of H2O2 and NO in the range from 10 to 300 mu M and 10 to 1100 mu M with a detection limit of 4 and 5 mu M, respectively. This MoS2-based biosensor was sensitive, reproducible and stable, indicating that AuNPs@MoS2 nanocomposite maybe a promising platform to construct electrochemical sensors for chemical and biological molecules detection.

引用

页数：9

共 41 条

[1]

[Anonymous], MODERN POLAROGRAPHIC

[2] Neurodegenerative diseases and oxidative stress [J].