Sensing Amyloid-β Aggregation Using Luminescent Dipyridophenazine Ruthenium(II) Complexes

被引：120

作者：

Cook, Nathan P. ^{[1
]}

Torres, Veronica ^{[1
]}

Jain, Disha ^{[1
]}

Marti, Angel A. ^{[1
,2
]}

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77005 USA

[2] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 29期

关键词：

IMAGING MICROSCOPY; ALZHEIMERS-DISEASE; THIOFLAVIN-T; DNA; PROBE; SWITCH; CELL;

D O I：

10.1021/ja204656r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The aggregation of amyloid-beta (A beta) peptides has been associated with the onset of Alzheimer's disease. Here, we report the use of a luminescent dipyridophenazine ruthenium(II) complex to monitor A beta fibrillization. This complex is not photoluminescent in aqueous solution nor in the presence of monomeric A beta, but it presents a strong photoluminescence in the presence of A beta fibril aggregates. One of the advantages of this metal complex is its large Stokes shift (180 nm). Furthermore, the long-lived photoluminescence lifetime of this ruthenium complex allows its use for the detection of fibrillar proteins in the presence of short-lived fluorescent backgrounds, using time-gating technology. We will present evidence of the advantages of dipyridophenazine ruthenium (II) complexes for monitoring protein fibrillization in highly fluorescent media.

引用

页码：11121 / 11123

页数：3

共 23 条

[21] Ruthenium(II) Complex Enantiomers as Cellular Probes for Diastereomeric Interactions in Confocal and Fluorescence Lifetime Imaging Microscopy [J].