Blastomeres aggregation as an efficient alternative for trophoblast culture from porcine parthenogenetic embryos

被引：17

作者：

Saadeldin, Islam M. ^{[1
,2
,3
]}

Kim, Su Jin ^{[1
,2
]}

Lee, Byeong Chun ^{[1
,2
,4
]}

机构：

[1] Seoul Natl Univ, Coll Vet Med, Dept Theriogenol & Biotechnol, Seoul 151742, South Korea

[2] Seoul Natl Univ, Res Inst Vet Sci, Seoul 151742, South Korea

[3] Zagazig Univ, Fac Vet Med, Dept Physiol, Zagazig 44519, Egypt

[4] Seoul Natl Univ, Inst Green Bio Sci Technol, Designed Anim & Transplantat, Pyeongchang 232916, South Korea

来源：

DEVELOPMENT GROWTH & DIFFERENTIATION | 2015年 / 57卷 / 05期

关键词：

blastomeres aggregation; parthenogenesis; pig; trophoblast cells; INNER CELL MASS; STEM-CELLS; IN-VITRO; TROPHECTODERM; EXPRESSION; SPECIFICATION; BLASTOCYSTS; MATURATION;

D O I：

10.1111/dgd.12215

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Zona pellucida free (ZPF) oocytes were cultured after electrical activation to allow blastomeres aggregation and compared to ZP intact (ZPI) oocytes. In feeder-dependent conditions, the trophoblast attachment and primary outgrowths were significantly higher in ZPF than in ZPI groups. In feeder-free conditions, trophoblast attachment and typical morphological trophoblast primary outgrowths were observed in ZPF group. The primary colonies derived from the ZPF embryos in both culture conditions were able to establish secondary and tertiary colonies and showed mRNA expression of CDX2, TEAD4 and KRT8 as trophoblast markers, while outgrowths from the ZPI embryos could not grow beyond primary colonies.

引用

页码：362 / 368

页数：7

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