The adenovirus type 5 E1B-55K oncoprotein actively shuttles in virus-infected cells, whereas transport of E4orf6 is mediated by a CRM1-Independent mechanism

被引：44
作者：
Dosch, T
Horn, F
Schneider, G
Krätzer, F
Dobner, T
Hauber, J
Stauber, RH
机构：
[1] Univ Erlangen Nurnberg, Inst Clin & Mol Virol, D-91054 Erlangen, Germany
[2] Univ Regensburg, Inst Med Mikrobiol & Hyg, D-93053 Regensburg, Germany
来源：
JOURNAL OF VIROLOGY | 2001年 / 75卷 / 12期
关键词：
D O I：
10.1128/JVI.75.12.5677-5683.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved in viral mRNA export. Here we demonstrate that adenovirus infection does not inhibit the function of the E1B-55K nuclear export signal and that E1B-55K also shuttles in infected cells, Even during virus infection, E1B-55K was exported by the leptomycin B-sensitive CRM1 pathway, whereas E4orf6 transport appeared to be mediated by an alternative mechanism. Our results strengthen the potential role of E1B-55K as the "driving force" for adenoviral late mRNA export.
引用
收藏
页码：5677 / 5683
页数：7
相关论文
共 34 条
[21]  
PAVLAKIS GN, 1998, HUMAN IMMUNODEFICIEN, P103
[22]   Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway [J].
Rabino, C ;
Aspegren, A ;
Corbin-Lickfett, K ;
Bridge, E .
JOURNAL OF VIROLOGY, 2000, 74 (14) :6684-6688
[23]   MONOCLONAL-ANTIBODIES WHICH RECOGNIZE NATIVE AND DENATURED FORMS OF THE ADENOVIRUS DNA-BINDING PROTEIN [J].
REICH, NC ;
SARNOW, P ;
DUPREY, E ;
LEVINE, AJ .
VIROLOGY, 1983, 128 (02) :480-484
[24]   Direct observation of nucleocytoplasmic transport by microinjection of GFP-tagged proteins in living cells [J].
Rosorius, O ;
Heger, P ;
Stelz, G ;
Hirschmann, N ;
Hauber, J ;
Stauber, RH .
BIOTECHNIQUES, 1999, 27 (02) :350-+
[25]   Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex [J].
Rubenwolf, S ;
Schutt, H ;
Nevels, M ;
Wolf, H ;
Dobner, T .
JOURNAL OF VIROLOGY, 1997, 71 (02) :1115-1123
[26]   ADENOVIRUS EARLY REGION-1B 58,000-DALTON TUMOR-ANTIGEN IS PHYSICALLY ASSOCIATED WITH AN EARLY REGION-4 25,000-DALTON PROTEIN IN PRODUCTIVELY INFECTED-CELLS [J].
SARNOW, P ;
HEARING, P ;
ANDERSON, CW ;
HALBERT, DN ;
SHENK, T ;
LEVINE, AJ .
JOURNAL OF VIROLOGY, 1984, 49 (03) :692-700
[27]   ADENOVIRUS E1B-58KD TUMOR-ANTIGEN AND SV40 LARGE TUMOR-ANTIGEN ARE PHYSICALLY ASSOCIATED WITH THE SAME 54 KD CELLULAR PROTEIN IN TRANSFORMED-CELLS [J].
SARNOW, P ;
HO, YS ;
WILLIAMS, J ;
LEVINE, AJ .
CELL, 1982, 28 (02) :387-394
[28]  
Shenk T., 1996, Fields Virology, VThird, P2111
[29]   ANALYSIS OF TRAFFICKING OF REV AND TRANSDOMINANT REV PROTEINS IN LIVING CELLS USING GREEN FLUORESCENT PROTEIN FUSIONS - TRANSDOMINANT REV BLOCKS THE EXPORT OF REV FROM THE NUCLEUS TO THE CYTOPLASM [J].
STAUBER, R ;
GAITANARIS, GA ;
PAVLAKIS, GN .
VIROLOGY, 1995, 213 (02) :439-449
[30]   Intracellular trafficking and interactions of the HIV-1 Tat protein [J].
Stauber, RH ;
Pavlakis, GN .
VIROLOGY, 1998, 252 (01) :126-136
1234