The adenovirus type 5 E1B-55K oncoprotein actively shuttles in virus-infected cells, whereas transport of E4orf6 is mediated by a CRM1-Independent mechanism

被引：44
作者：
Dosch, T
Horn, F
Schneider, G
Krätzer, F
Dobner, T
Hauber, J
Stauber, RH
机构：
[1] Univ Erlangen Nurnberg, Inst Clin & Mol Virol, D-91054 Erlangen, Germany
[2] Univ Regensburg, Inst Med Mikrobiol & Hyg, D-93053 Regensburg, Germany
来源：
JOURNAL OF VIROLOGY | 2001年 / 75卷 / 12期
关键词：
D O I：
10.1128/JVI.75.12.5677-5683.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved in viral mRNA export. Here we demonstrate that adenovirus infection does not inhibit the function of the E1B-55K nuclear export signal and that E1B-55K also shuttles in infected cells, Even during virus infection, E1B-55K was exported by the leptomycin B-sensitive CRM1 pathway, whereas E4orf6 transport appeared to be mediated by an alternative mechanism. Our results strengthen the potential role of E1B-55K as the "driving force" for adenoviral late mRNA export.
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页码：5677 / 5683
页数：7
相关论文
共 34 条
[1]  
Amirand C, 1998, J CELL SCI, V111, P3551
[2]   MAJOR NUCLEOLAR PROTEINS SHUTTLE BETWEEN NUCLEUS AND CYTOPLASM [J].
BORER, RA ;
LEHNER, CF ;
EPPENBERGER, HM ;
NIGG, EA .
CELL, 1989, 56 (03) :379-390
[3]   REDUNDANT CONTROL OF ADENOVIRUS LATE GENE-EXPRESSION BY EARLY REGION-4 [J].
BRIDGE, E ;
KETNER, G .
JOURNAL OF VIROLOGY, 1989, 63 (02) :631-638
[4]   Diffusion of proteins across the nuclear envelope of HeLa cells [J].
Chatterjee, S ;
Stochaj, U .
BIOTECHNIQUES, 1998, 24 (04) :668-674
[5]   Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence [J].
Dobbelstein, M ;
Roth, J ;
Kimberly, WT ;
Levine, AJ ;
Shenk, T .
EMBO JOURNAL, 1997, 16 (14) :4276-4284
[6]   HNS, a nuclear-cytoplasmic shuttling sequence in HuR [J].
Fan, XHC ;
Steitz, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15293-15298
[7]   A potential NES of the Epstein-Barr virus nuclear antigen 1 (EBNA1) does not confer shuttling [J].
Fischer, N ;
Voss, MD ;
Muller-Lantzsch, N ;
Grässer, FA .
FEBS LETTERS, 1999, 447 (2-3) :311-314
[8]   CRM1 is an export receptor for leucine-rich nuclear export signals [J].
Fornerod, M ;
Ohno, M ;
Yoshida, M ;
Mattaj, IW .
CELL, 1997, 90 (06) :1051-1060
[9]   ADENOVIRUS EARLY REGION-4 ENCODES FUNCTIONS REQUIRED FOR EFFICIENT DNA-REPLICATION, LATE GENE-EXPRESSION, AND HOST-CELL SHUTOFF [J].
HALBERT, DN ;
CUTT, JR ;
SHENK, T .
JOURNAL OF VIROLOGY, 1985, 56 (01) :250-257
[10]   Titration of cellular export factors, but not heteromultimerization, is the molecular mechanism of trans-dominant HTLV-1 Rex mutants [J].
Heger, P ;
Rosorius, O ;
Hauber, J ;
Stauber, RH .
ONCOGENE, 1999, 18 (28) :4080-4090
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