NMR detection of bifurcated hydrogen bonds in large proteins

被引：24

作者：

Liu, Aizhuo ^{[1
]}

Lu, Zhenwei ^{[1
]}

Wang, Jifeng ^{[1
]}

Yao, Lishan ^{[1
,2
]}

Li, Yue ^{[1
]}

Yan, Honggao ^{[1
]}

机构：

[1] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[2] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 08期

关键词：

D O I：

10.1021/ja710114r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hydrogen bonds play critical roles in protein structure, stability, and function. Conventionally, hydrogen bonds are mainly determined by X-ray crystallography and NOE-based NMR spectroscopy in indirect manners. In recent years, it was demonstrated that hydrogen bonds can be directly detected through NMR measurements of trans-hydrogen-bond scalar coupling constants. Here we report across hydrogen-bond protium/deuterium isotope effects in a 35 kDA protein observed with the isotopomer-selective TROSY NMR technique (Liu at al. J. Biomol. NMR 2006, 36, 205-214; Liu at al. J. Magn. Reson. 2007, 186, 319-326) and show that such isotope effects can be used to detect a most common type of bifurcated hydrogen bonds, in which a heavy atom, usually oxygen, is involved in two hydrogen bonds, including a pair of bifurcated hydrogen bonds involving a bound water molecule.

引用

页码：2428 / 2429

页数：2

共 22 条

[1] Isotope effects on chemical shifts as an analytical tool in structural studies of intramolecular hydrogen bonded compounds [J].

Bolvig, S ;

Hansen, PE .

CURRENT ORGANIC CHEMISTRY, 2000, 4 (01) :19-54

[2] Insights into biomolecular hydrogen bonds from hydrogen bond scalar couplings [J].

Grzesiek, S ;

Cordier, F ;

Jaravine, V ;

Barfield, M .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2004, 45 (3-4) :275-300

[3]

Hansen PE, 2000, MAGN RESON CHEM, V38, P1

[4] DEUTERIUM AND O-18 ISOTOPE EFFECTS ON C-13 CHEMICAL-SHIFTS OF STERICALLY HINDERED AND/OR INTRAMOLECULARLY HYDROGEN-BONDED O-HYDROXY ACYL AROMATICS [J].

HANSEN, PE ;

IBSEN, SN ;

KRISTENSEN, T ;

BOLVIG, S .

MAGNETIC RESONANCE IN CHEMISTRY, 1994, 32 (07) :399-408

[5]

Harris TK, 1999, PROTEINS, V35, P275, DOI 10.1002/(SICI)1097-0134(19990515)35:3<275::AID-PROT1>3.0.CO

[6]

2-V

[7]

Hibbert F., 1990, ADV PHYS ORG CHEM, V26, P255, DOI DOI 10.1016/S0065-3160(08)60047-7

[8] The 1.14 Å crystal structure of yeast cytosine deaminase:: Evolution of nucleotide salvage enzymes and implications for genetic chemotherapy [J].

Ireton, GC ;

Black, ME ;

Stoddard, BL .

STRUCTURE, 2003, 11 (08) :961-972

[9] Clinical development of gene therapy for colorectal cancer [J].

Kerr, D .

NATURE REVIEWS CANCER, 2003, 3 (08) :615-622

[10] Computational and empirical trans-hydrogen bond deuterium isotope shifts suggest that N1-N3 A:U hydrogen bonds of RNA are shorter than those of A:T hydrogen bonds of DNA [J].

Kim, YI ;

Manalo, MN ;

Peréz, LM ;

LiWang, A .

JOURNAL OF BIOMOLECULAR NMR, 2006, 34 (04) :229-236

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