Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells

被引：75

作者：

Youn, Myung-A ^{[1
]}

Kim, Jin-Kyung ^{[1
]}

Park, Seong-Yeol ^{[1
]}

Kim, Yunha ^{[1
]}

Kim, Se-Jin ^{[1
]}

Lee, Jin Seok ^{[3
]}

Chai, Kyu Yun ^{[3
]}

Kim, Hye-Jung ^{[4
]}

Cui, Ming-Xun ^{[2
]}

So, Hong Seob ^{[1
]}

Kim, Ki-Young ^{[2
]}

Park, Raekil ^{[1
]}

机构：

[1] Wonkwang Univ, Sch Med, Vestibulocochlear Res Ctr, Iksan 570749, Jeonbuk, South Korea

[2] Wonkwang Univ, Coll Human Environm Sci, Jeonbuk, South Korea

[3] Wonkwang Univ, Dept Bionanochem, Coll Nat Sci, Jeonbuk, South Korea

[4] Wonkwang Univ, Wonkwang Univ Hosp, Dept Family Med, Jeonbuk, South Korea

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2008年 / 14卷 / 04期

关键词：

Inonotus obliquus; cell cycle; G(0)/G(1) arrest; apoptosis;

D O I：

10.3748/wjg.14.511

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines, HepG2 and Hep3B cells. METHODS: The cytotoxicity of Chaga extract was screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract. RESULTS: HepG2 cells were more sensitive to Chaga extract than Hep3B cells, as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner, which was accompanied with G(0)/G(1)-phase arrest and apoptotic cell death. In addition, G(0)/G(1) arrest in the cell cycle was closely associated with down-regulation of p53, pRb, p27, cyclins D1, D2, E, cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 expression. CONCLUSION: Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma. (c) 2008 WJG. All rights reserved.

引用

页码：511 / 517

页数：7

共 31 条

[1] Grape seed extract induces apoptotic death of human prostate carcinoma DU145 cells via caspases activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release [J].

Agarwal, C ;

Singh, RP ;

Agarwal, R .

CARCINOGENESIS, 2002, 23 (11) :1869-1876

[2] Mushrooms, tumors, and immunity [J].

Borchers, AT ;

Stern, JS ;

Hackman, RM ;

Keen, CL ;

Gershwin, ME .

PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1999, 221 (04) :281-293

[3] ABNORMAL STRUCTURE AND EXPRESSION OF P53 GENE IN HUMAN HEPATOCELLULAR-CARCINOMA [J].

BRESSAC, B ;

GALVIN, KM ;

LIANG, TJ ;

ISSELBACHER, KJ ;

WANDS, JR ;

OZTURK, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) :1973-1977

[4] Opposing securin and p53 protein expression in the oxaliplatin-induced cytotoxicity of human colorectal cancer cells [J].

Chiu, Shu-Jun ;

Hsu, Tzu-Sheng ;

Chao, Jui-I .

TOXICOLOGY LETTERS, 2006, 167 (02) :122-130

[5] PI3K: Downstream AKTion blocks apoptosis [J].

Franke, TF ;

Kaplan, DR ;

Cantley, LC .

CELL, 1997, 88 (04) :435-437

[6]

Hall M, 1996, ADV CANCER RES, V68, P67, DOI 10.1016/S0065-230X(08)60352-8

[7] p53: 25 years after its discovery [J].

Hofseth, LJ ;

Hussain, SP ;

Harris, CC .

TRENDS IN PHARMACOLOGICAL SCIENCES, 2004, 25 (04) :177-181

[8] HIV-1 protease inhibition and anti-HIV effect of natural and synthetic water-soluble lignin-like substances [J].

Ichimura, T ;

Otake, T ;

Mori, H ;

Maruyama, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (12) :2202-2204

[9]

KAHLOS K, 1986, PLANTA MED, P554

[10] Anti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia of Inonotus obliquus [J].

Kim, Yong Ook ;

Park, Hae Woong ;

Kim, Jong Hoon ;

Lee, Jae Young ;

Moon, Seong Hoon ;

Shin, Chul Soo .

LIFE SCIENCES, 2006, 79 (01) :72-80

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