Covalently bonded polysaccharide-modified stationary phase for per aqueous liquid chromatography and hydrophilic interaction chromatography

被引：31

作者：

Li, Yuanyuan ^{[1
,2
]}

Li, Jiao ^{[1
]}

Chen, Tong ^{[1
]}

Liu, Xiaoyan ^{[1
]}

Zhang, Haixia ^{[1
]}

机构：

[1] Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

[2] Ningxia Univ, Key Lab Energy & Chem Engn, Yinchuan 750021, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Polysaccharide; HILIC; PALC; Stationary phase; Green chromatography; HIGHLY POLAR; PERFORMANCE; SEPARATION; WATER; SELECTIVITY; COLUMN;

D O I：

10.1016/j.chroma.2011.01.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The mixed sulfated/methacryloyl polysaccharide derivative was prepared and successfully immobilized onto the surface of porous silica particles by polymerization. Polysaccharide derivative was calculated as 10.33% in the stationary phase prepared. The new stationary phase (PMSP) showed both hydrophilic interaction (HILIC) and per aqueous liquid chromatography (PALC) characteristics. The effects of column temperature, the water content, pH and ion strength of mobile phase on the retention time of test compounds in highly aqueous eluents were investigated to evaluate the PALC features of PMSP. The column efficiency is about 31,000 plates/m for benzoic acid in water/ACN (97/3, v/v) mobile phase at a flow rate of 1.0 mL/min. Compared with C18 column, the PMSP had shorter retention time for weak polar and non-polar compounds, but also showed stronger retention for strong polar compounds. It indicated that PALC was a suitable mode of chromatography as replacement of HILIC and complementarity of reversed-phase liquid chromatography (RPLC). (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1503 / 1508

页数：6

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