Genetic heterogeneity in Mycobacterium tuberculosis isolates reflected IS6110 restriction fragment length polymorphism patterns as low-intensity bands

被引:52
作者
de Boer, AS
Kremer, K
Borgdorff, MW
de Haas, PEW
Heersma, HF
van Soolingen, D
机构
[1] Natl Inst Publ Hlth & Environm, Dept Infect Dis Epidemiol, NL-3720 BA Bilthoven, Netherlands
[2] Natl Inst Publ Hlth & Environm, Lab Infect Dis & Perinatal Screening, Dept Mycobacteriol, NL-3720 BA Bilthoven, Netherlands
[3] Natl Inst Publ Hlth & Environm, Staff Bur Informat & Methodol Advice, NL-3720 BA Bilthoven, Netherlands
[4] Royal Netherlands TB Assoc, NL-2501 CC The Hague, Netherlands
关键词
D O I
10.1128/JCM.38.12.4478-4484.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mycobacterium tuberculosis isolates with identical IS6110 restriction fragment length polymorphism (RFLP) patterns are considered to originate from the same ancestral strain and thus to reflect ongoing transmission. Zn this study, we investigated 1,277 IS6110 RFLP patterns for the presence of multiple low-intensity bands (LLBs), which may indicate infections with multiple M. tuberculosis strains. We did not find any multiple LIBs, suggesting that multiple infections are rare in the Netherlands. However, we did observe 3 few LIBs in 94 patterns terns (7.4%) and examined the nature of this phenomenon. With single-colony cultures it was found that LIBs mostly represent mixed bacterial populations with slightly different RFLP patterns, Mixtures were expressed in RFLP patterns as LIBs when 10 to 30% Of the DNA analyzed originated from a bacterial population with another RFLP pattern. presumably, a part of the LIBs did not represent mixed bacterial populations, as in some clusters all strains exhibited LLBs in their RFLP patterns. The occurrence of LIBs was associated with increased age in patients. This may reflect either a gradual change of the bacterial population in the human body over time or IS6110-mediated genetic adaptation of ni. tuberculosis to changes in the environmental conditions during the dormant state or reactivation thereafter.
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页码:4478 / 4484
页数:7
相关论文
共 34 条
  • [1] Transmission of tuberculosis among the urban homeless
    Barnes, PF
    ElHajj, H
    PrestonMartin, S
    Cave, MD
    Jones, BE
    Otaya, M
    Pogoda, J
    Eisenach, KD
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1996, 275 (04): : 305 - 307
  • [2] Results from 5 years of nationwide DNA fingerprinting of Mycobacterium tuberculosis complex isolates in a country with a low incidence of M-tuberculosis infection
    Bauer, J
    Yang, ZH
    Poulsen, S
    Andersen, ÅB
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) : 305 - 308
  • [3] Borgdorff MW, 1998, AM J EPIDEMIOL, V147, P187
  • [4] The application of molecular techniques to the diagnosis and epidemiology of mycobacterial diseases
    Butcher, PD
    Hutchinson, NA
    Doran, TJ
    Dale, JW
    [J]. JOURNAL OF APPLIED BACTERIOLOGY, 1996, 81 : S53 - S71
  • [5] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
    Cole, ST
    Brosch, R
    Parkhill, J
    Garnier, T
    Churcher, C
    Harris, D
    Gordon, SV
    Eiglmeier, K
    Gas, S
    Barry, CE
    Tekaia, F
    Badcock, K
    Basham, D
    Brown, D
    Chillingworth, T
    Connor, R
    Davies, R
    Devlin, K
    Feltwell, T
    Gentles, S
    Hamlin, N
    Holroyd, S
    Hornby, T
    Jagels, K
    Krogh, A
    McLean, J
    Moule, S
    Murphy, L
    Oliver, K
    Osborne, J
    Quail, MA
    Rajandream, MA
    Rogers, J
    Rutter, S
    Seeger, K
    Skelton, J
    Squares, R
    Squares, S
    Sulston, JE
    Taylor, K
    Whitehead, S
    Barrell, BG
    [J]. NATURE, 1998, 393 (6685) : 537 - +
  • [6] TRANSMISSION OF MULTIDRUG-RESISTANT MYCOBACTERIUM-TUBERCULOSIS AMONG PERSONS WITH HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION IN AN URBAN HOSPITAL - EPIDEMIOLOGIC AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS
    CORONADO, VG
    BECKSAGUE, CM
    HUTTON, MD
    DAVIS, BJ
    NICHOLAS, P
    VILLAREAL, C
    WOODLEY, CL
    KILBURN, JO
    CRAWFORD, JT
    FRIEDEN, TR
    SINKOWITZ, RL
    JARVIS, WR
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (04) : 1052 - 1055
  • [7] AN OUTBREAK OF TUBERCULOSIS WITH ACCELERATED PROGRESSION AMONG PERSONS INFECTED WITH THE HUMAN-IMMUNODEFICIENCY-VIRUS - AN ANALYSIS USING RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS
    DALEY, CL
    SMALL, PM
    SCHECTER, GF
    SCHOOLNIK, GK
    MCADAM, RA
    JACOBS, WR
    HOPEWELL, PC
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (04) : 231 - 235
  • [8] IS6110 restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, South India
    Das, S
    Paramasivan, CN
    Lowrie, DB
    Prabhakar, R
    Narayanan, PR
    [J]. TUBERCLE AND LUNG DISEASE, 1995, 76 (06): : 550 - 554
  • [9] Analysis of rate of change of IS6110 RFLP patterns of Mycobacterium tuberculosis based on serial patient isolates
    de Boer, AS
    Borgdorff, MW
    de Haas, PEW
    Nagelkerke, NJD
    van Embden, JDA
    van Soolingen, D
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (04) : 1238 - 1244
  • [10] DESPREZ R, 1990, PRINCIPLES PRACTICE, P1880