DNA barcoding of faeces or stomach contents is an emerging approach for dietary analysis. We pyrosequenced mtDNA 16S markers amplified from faeces of captive little penguins (Eudyptula minor) to examine if recovered sequences reflect the proportions of species consumed. We also analysed wild little penguin faeces collected from 100 nests in southeast Australia. In the captive study, pilchards were the primary fish fed to the penguins and DNA sequences from pilchard were the most common sequences recovered. Sequences of three other fish fed in constant mass proportions (45:35:20) were all detected, but proportions of sequences (60:6:34) were considerably different than mass proportions in the diet. Correction factors based on relative mtDNA density in the fish did not improve diet estimates. Consistency between replicate samples suggests that the observed bias resulted from differences in prey digestibility. Detection of DNA from fish consumed before the penguins were brought into captivity indicates that a DNA signal in faeces can persist for at least 4 days after ingestion. In the wild-collected faeces, 24 distinct fish and 1 squid were identified; anchovy, barracouta and pilchard accounted for over 80% of these sequences. Our results highlight that DNA sequences recovered in dietary barcoding studies can provide semi-quantitative information on diet composition, but these data should be given wide confidence intervals.
机构:
Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Porazinska, Dorota L.
Giblin-Davis, Robin M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Giblin-Davis, Robin M.
Faller, Lina
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Faller, Lina
Farmerie, William
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32608 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Farmerie, William
Kanzaki, Natsumi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Forestry & Forest Prod Res Inst, Forest Pathol Lab, Tsukuba, Ibaraki 3058687, JapanUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Kanzaki, Natsumi
Morris, Krystalynne
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Morris, Krystalynne
Powers, Thomas O.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Powers, Thomas O.
Tucker, Abraham E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Tucker, Abraham E.
Sung, Way
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Sung, Way
Thomas, W. Kelley
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
机构:
Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Porazinska, Dorota L.
Giblin-Davis, Robin M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Giblin-Davis, Robin M.
Faller, Lina
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Faller, Lina
Farmerie, William
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32608 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Farmerie, William
Kanzaki, Natsumi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Forestry & Forest Prod Res Inst, Forest Pathol Lab, Tsukuba, Ibaraki 3058687, JapanUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Kanzaki, Natsumi
Morris, Krystalynne
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Morris, Krystalynne
Powers, Thomas O.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Powers, Thomas O.
Tucker, Abraham E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Tucker, Abraham E.
Sung, Way
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA
Sung, Way
Thomas, W. Kelley
论文数: 0引用数: 0
h-index: 0
机构:
Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USAUniv Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA