Oligonucleotide-Hybridization Fiber-Optic Biosensor Using a Narrow Bandwidth Long Period Grating

被引:17
作者
Delgado-Pinar, Martina [1 ]
Shi, Qing [2 ]
Poveda-Wong, Luis [1 ]
Delgado-Pinar, Estefania [3 ]
Xu, Baojian [2 ]
Zhao, Jianlog [2 ]
Luis Cruz, Jose [1 ]
Andres, Miguel V. [1 ]
机构
[1] Univ Valencia, Dept Fis Aplicada & Electromagnetismo ICMUV, E-46100 Valencia, Spain
[2] Shanghai Tech Univ, Univ Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
[3] Univ Valencia, ICMOL, C Catedrat Jose Beltran 2, Paterna 46980, Spain
关键词
Fiber optics; fiber optics sensors; fiber gratings; biosensors; CHEMICAL SENSORS; INDEX;
D O I
10.1109/JSEN.2017.2723759
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Nanometric narrowband long period gratings (LPGs) are investigated for the implementation of improved fiber optic biosensors. The reduction of more than one order of magnitude in the linewidth of the LPG with respect to standard LPG at 1500 nm leads to the improvement of the resolution of the sensor. By selecting the proper fabrication parameters (high numerical aperture, relatively high order mode, and large length), LPGs with a 3-dB bandwidth of 1.5 nm were fabricated. The sensitivity of the LPG as a refractometer was calculated and experimentally characterized in detail. In particular, the LP0,17 and LP0,18 resonances were investigated in order to select the most suitable one for the bioexperiments. The surface of the LPG was functionalized and the detection of the hybridization of DNA is demonstrated. When the biosensor was immersed in a 2 mu M solution of the complementary DNA strand, the resonances of the LPG shifted in wavelength. When measuring the response of the sensor in terms of variation of its transmittance as the resonance shifted in wavelength, the sensitivity was similar to 10%/mu M for both resonances. The detection limit was estimated in 10 nM.
引用
收藏
页码:5503 / 5509
页数:7
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