Diazirine-containing RNA photocrosslinking probes for the study of siRNA-protein interactions

被引：16

作者：

Kuboe, Satoru ^{[2
]}

Yoda, Mayuko ^{[1
,4
]}

Ogata, Aya ^{[2
]}

Kitade, Yukio ^{[2
,3
]}

Tomari, Yukihide ^{[1
,4
]}

Ueno, Yoshihito ^{[2
,3
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Tokyo 1130032, Japan

[2] Gifu Univ, Dept Biomol Sci, Fac Engn, Gifu 5011193, Japan

[3] Gifu Univ, United Grad Sch Drug Discovery & Med Informat Sci, Gifu 5011193, Japan

[4] Univ Tokyo, Dept Med Genome Sci, Tokyo 1130032, Japan

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 39期

基金：

日本科学技术振兴机构;

关键词：

DROSOPHILA; RISC; INTERFERENCE; CLEAVES; STRAND;

D O I：

10.1039/c0cc02450c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3'-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.

引用

页码：7367 / 7369

页数：3

共 20 条

[1] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[2] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[3] 3-Azidodifluoromethyl-3H-diazirin-3-yl group as an all-in-one functional group for radioisotope-free photoafiinity labeling [J].

Hiramatsu, Toshiyuki ;

Guo, Ying ;

Hosoya, Takamitsu .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (18) :2916-2919

[4] Hsc70/Hsp90 Chaperone Machinery Mediates ATP-Dependent RISC Loading of Small RNA Duplexes [J].

Iwasaki, Shintaro ;

Kobayashi, Maki ;

Yoda, Mayuko ;

Sakaguchi, Yuriko ;

Katsuma, Susumu ;

Suzuki, Tsutomu ;

Tomari, Yukihide .

MOLECULAR CELL, 2010, 39 (02) :292-299

[5] Making RISC [J].

Kawamata, Tomoko ;

Tomari, Yukihide .

TRENDS IN BIOCHEMICAL SCIENCES, 2010, 35 (07) :368-376

[6] R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway [J].

Liu, QH ;

Rand, TA ;

Kalidas, S ;

Du, FH ;

Kim, HE ;

Smith, DP ;

Wang, XD .

SCIENCE, 2003, 301 (5641) :1921-1925

[7] Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes [J].

Matranga, C ;

Tomari, Y ;

Shin, C ;

Bartel, DP ;

Zamore, PD .

CELL, 2005, 123 (04) :607-620

[8] Slicer function of Drosophila Argonautes and its involvement in RISC formation [J].

Miyoshi, K ;

Tsukumo, H ;

Nagami, T ;

Siomi, H ;

Siomi, MC .

GENES & DEVELOPMENT, 2005, 19 (23) :2837-2848

[9] A Dicer-2-dependent 80S complex cleaves targeted mRNAs during RNAi in Drosophila [J].

Pham, JW ;

Pellino, JL ;

Lee, YS ;

Carthew, RW ;

Sontheimer, EJ .

CELL, 2004, 117 (01) :83-94

[10] A Diazirine-Based Nucleoside Analogue for Efficient DNA Interstrand Photocross-Linking [J].

Qiu, Zhihai ;

Lu, Lianghua ;

Jian, Xing ;

He, Chuan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (44) :14398-+

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