Improvement of sciatic nerve regeneration by multichannel nanofibrous membrane-embedded electro-conductive conduits functionalized with laminin

被引：13

作者：

Nazeri, Niloofar ^{[1
,2
]}

Derakhshan, Mohammad Ali ^{[3
]}

Mansoori, Korosh ^{[4
]}

Ghanbari, Hossein ^{[2
,5
]}

机构：

[1] Qazvin Univ Med Sci, Cellular & Mol Res Ctr, Qazvin, Iran

[2] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Med Nanotechnol, Tehran, Iran

[3] Shiraz Univ Med Sci, Sch Adv Med Sci & Technol, Dept Med Nanotechnol, Shiraz, Iran

[4] Iran Univ Med Sci, Neuromusculoskeletal Res Ctr, Tehran, Iran

[5] Univ Tehran Med Sci, Cardiovasc Dis Res Inst, Res Ctr Adv Technol Cardiovasc Med, Tehran, Iran

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2022年 / 33卷 / 06期

关键词：

Electrospinning; PLGA; CNT; Nanofiber; Nerve conduit; ELECTROSPUN FIBERS; CONDUCTIVE CONDUIT; NEURITE OUTGROWTH; SCHWANN-CELLS; STEM-CELLS; SCAFFOLD; MUSCLE; DIFFERENTIATION; POLYURETHANE; ENHANCEMENT;

D O I：

10.1007/s10856-022-06669-0

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Multichannel structures in the design of nerve conduits offer potential advantages for regeneration of damaged nerves. However, lack of biochemical cues and electrical stimulation could hamper satisfactory nerve regeneration. The aim of this study was to simultaneously evaluate the effects of topographical, biological, and electrical cues on sciatic nerve regeneration. Accordingly, a series of multichannel nerve conduit was made using longitudinally-aligned laminin-coated poly (lactic-co-glycolic acid) (PLGA)/carbon nanotubes (CNT) nanofibers (NF, mean diameter: 455 +/- 362 nm) in the lumen and randomly-oriented polycaprolactone (PCL) NF (mean diameter: 340 +/- 200 nm) on the outer surface. In vitro studies revealed that the materials were nontoxic and able to promote cell attachment and proliferation on nanofibers and on fibrin gel. To determine the influence of laminin as biological and CNT as electrical cues on nerve regeneration, either of hollow PCL conduits, PLGA NF-embedded, PLGA/CNT NF-embedded or laminin-coated PLGA/CNT NF-embedded PCL conduits were implanted in rats. A new surgery method was utilized and results were compared with an autograft. The results of motor and sensory tests in addition to histopathological examination of the regenerated nerves demonstrated the formation of nerve fibers in laminin-coated PLGA/CNT NF-embedded PCL conduits. Results suggested that these conduits have the potential to improve sciatic nerve regeneration. [GRAPHICS] .

引用

页数：16