Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana

被引：86

作者：

Annan, Kofi ^{[2
]}

Houghton, Peter J. ^{[1
]}

机构：

[1] Kings Coll London, Pharmaceut Sci Res Div, Pharmacognosy Res Labs, London SE1 9NH, England

[2] Kwame Nkrumah Univ Sci & Technol, Coll Hlth Sci, Fac Pharm & Pharmaceut Sci, Kumasi, Ghana

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2008年 / 119卷 / 01期

关键词：

Ficus asperifolia; Gossypium arboreum; antibacterial; antioxidant; fibroblast;

D O I：

10.1016/j.jep.2008.06.017

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aim of the study: Use of in vitro tests to search for relevant activities in bark of Ficus asperifolia Miq. and leaves of Gossypium arboreum L, used in Ghana for wound healing. Materials and methods: Aqueous extracts of the relevant parts of the two species were tested for antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Micrococcus flavus, Escherichia coli, Pseudomonas aeruginosa and resistant strains of Staphylococcus aureus SA1199B, RN4220 and XU212. The effects of the plants on fibroblast growth stimulation as well as antioxidant protective effect against hydrogen peroxide induced damage in the same cell line were also studied. Results: The extracts of Gossypium arboreum and Ficus asperifolia had weak antibacterial action against all bacteria tested. The extracts of the two plants had significant (p < 0.001) effects on the growth of human dermal fibroblast at 50 mu g/ml and lower. They also significantly protected fibroblast cells against oxidative damage at doses up to 50 mu g/ml. Gossypium arboreum leaf extract displayed the higher activity. Conclusion: The stimulatory effect on fibroblast growth and protection against peroxide-induced oxidative damage give some support to the traditional use of these two plants as wound-healing agents. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：141 / 144

页数：4

共 20 条

[1]

Abbiw D., 1990, USEFUL PLANTS GHANA, P182

[2]

Annan K, 2007, THESIS U LONDON UK, P30

[3]

Bizimenyera ES, 2007, AFR J TRADIT COMPLEM, V4, P99

[4]

BODEKER G, 1998, PLANTS FOOD MED

[5] Treatment with supplementary arginine, vitamin C and zinc in patients with pressure ulcers: A randomised controlled trial [J].

Desneves, KJ ;

Todorovic, BE ;

Cassar, A ;

Crowe, TC .

CLINICAL NUTRITION, 2005, 24 (06) :979-987

[6] A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria [J].

Eloff, JN .

PLANTA MEDICA, 1998, 64 (08) :711-713

[7] In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride [J].

Fotakis, G ;

Timbrell, JA .

TOXICOLOGY LETTERS, 2006, 160 (02) :171-177

[8] Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries [J].

Gyamfi, MA ;

Yonamine, M ;

Aniya, Y .

GENERAL PHARMACOLOGY, 1999, 32 (06) :661-667

[9] In vitro tests and ethnopharmacological investigations: Wound healing as an example [J].

Houghton, PJ ;

Hylands, PJ ;

Mensah, AY ;

Hensel, A ;

Deters, AM .

JOURNAL OF ETHNOPHARMACOLOGY, 2005, 100 (1-2) :100-107

[10]

Mensah AY, 2004, J PHARM PHARMACOL, V56, pS79

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