Molecular approaches for AM fungal community ecology: A primer

被引：26

作者：

Gorzelak, Monika A. ^{[1
]}

Holland, Taylor C. ^{[1
]}

Xing, Xiaoke ^{[2
,3
]}

Hart, Miranda M. ^{[1
]}

机构：

[1] Univ British Columbia Okanagan, Kelowna, BC V1V 1V7, Canada

[2] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[3] Peking Union Med Coll, Beijing 100193, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 90卷 / 02期

关键词：

Arbuscular mycorrhizas; Molecular biology; AM fungi; Soil ecology; High-throughput sequencing; Community profiling; ARBUSCULAR MYCORRHIZAL FUNGI; REAL-TIME PCR; RIBOSOMAL DNA; PLANT PRODUCTIVITY; COLONIZING ROOTS; IN-VIVO; SOIL; DIVERSITY; IDENTIFICATION; EXTRACTION;

D O I：

10.1016/j.mimet.2012.04.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular techniques are no longer optional for ecologists interested in arbuscular mycorrhizal (AM) communities. Understanding the role of these soil fungi in natural systems requires knowledge of their abundance and identity but this is impossible to achieve without a molecular approach. Adapting molecular tools to AM fungi can be challenging because of the unique biology of the fungi. Moreover, many recruits in the field of mycorrhizal ecology have little or no experience with molecular biology. Here, we outline a conceptual framework for designing robust ecological experiments with AM fungi using molecular approaches. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：108 / 114

页数：7

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