Molecular approaches for AM fungal community ecology: A primer

被引：26

作者：

Gorzelak, Monika A. ^{[1
]}

Holland, Taylor C. ^{[1
]}

Xing, Xiaoke ^{[2
,3
]}

Hart, Miranda M. ^{[1
]}

机构：

[1] Univ British Columbia Okanagan, Kelowna, BC V1V 1V7, Canada

[2] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[3] Peking Union Med Coll, Beijing 100193, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 90卷 / 02期

关键词：

Arbuscular mycorrhizas; Molecular biology; AM fungi; Soil ecology; High-throughput sequencing; Community profiling; ARBUSCULAR MYCORRHIZAL FUNGI; REAL-TIME PCR; RIBOSOMAL DNA; PLANT PRODUCTIVITY; COLONIZING ROOTS; IN-VIVO; SOIL; DIVERSITY; IDENTIFICATION; EXTRACTION;

D O I：

10.1016/j.mimet.2012.04.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular techniques are no longer optional for ecologists interested in arbuscular mycorrhizal (AM) communities. Understanding the role of these soil fungi in natural systems requires knowledge of their abundance and identity but this is impossible to achieve without a molecular approach. Adapting molecular tools to AM fungi can be challenging because of the unique biology of the fungi. Moreover, many recruits in the field of mycorrhizal ecology have little or no experience with molecular biology. Here, we outline a conceptual framework for designing robust ecological experiments with AM fungi using molecular approaches. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：108 / 114

页数：7

共 105 条

[1] INFECTIVITY OF VESICULAR ARBUSCULAR MYCORRHIZAL FUNGI - INFLUENCE OF HOST SOIL DILUENT COMBINATIONS ON MPN ESTIMATES AND PERCENTAGE COLONIZATION [J].

ADELMAN, MJ ;

MORTON, JB .

SOIL BIOLOGY & BIOCHEMISTRY, 1986, 18 (01) :77-83

[2] Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques [J].

Anderson, IC ;

Cairney, JWG .

ENVIRONMENTAL MICROBIOLOGY, 2004, 6 (08) :769-779

[3] Nuclei of symbiotic arbuscular mycorrhizal fungi as revealed by in vivo two-photon microscopy [J].

Bago, B ;

Zipfel, W ;

Williams, RM ;

Piché, Y .

PROTOPLASMA, 1999, 209 (1-2) :77-89

[4] In vivo studies on the nuclear behavior of the arbuscular mycorrhizal fungus Gigaspora rosea grown under axenic conditions [J].

Bago, B ;

Zipfel, W ;

Williams, RM ;

Chamberland, H ;

Lafontaine, JG ;

Webb, WW ;

Piché, Y .

PROTOPLASMA, 1998, 203 (1-2) :1-15

[5] Differential effect of sample preservation methods on plant and arbuscular mycorrhizal fungal DNA [J].

Bainard, L. D. ;

Klironomos, J. N. ;

Hart, M. M. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2010, 82 (02) :124-130

[6]

Berthelet M, 1996, FEMS MICROBIOL LETT, V138, P17

[7] Mechanism of retarded DNA degradation and prokaryotic origin of DNases in nonsterile soils [J].

Blum, SAE ;

Lorenz, MG ;

Wackernagel, W .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1997, 20 (04) :513-521

[8] Diversity of mitochondrial large subunit rDNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi-natural grasslands [J].

Boerstler, Boris ;

Thiery, Odile ;

Sykorova, Zuzana ;

Berner, Alfred ;

Redecker, Dirk .

MOLECULAR ECOLOGY, 2010, 19 (07) :1497-1511

[9] Rhizosphere communication of plants, parasitic plants and AM fungi [J].

Bouwmeester, Harro J. ;

Roux, Christophe ;

Lopez-Raez, Juan Antonio ;

Becard, Guillaume .

TRENDS IN PLANT SCIENCE, 2007, 12 (05) :224-230

[10] The elimination of primer-dimer accumulation in PCR [J].

Brownie, J ;

Shawcross, S ;

Theaker, J ;

Whitcombe, D ;

Ferrie, R ;

Newton, C ;

Little, S .

NUCLEIC ACIDS RESEARCH, 1997, 25 (16) :3235-3241

← 1 2 3 4 5 6 7 8 9 10 →