Elucidation of a complete mechanical signaling and virulence activation pathway in enterohemorrhagic Escherichia coli

被引：18

作者：

Feng, Lu ^{[1
,2
,3
]}

Yang, Bin ^{[1
,2
]}

Xu, Yongchang ^{[1
,2
]}

Xiong, Yingying ^{[1
,2
]}

Wang, Fang ^{[1
,2
]}

Liu, Bin ^{[1
,2
]}

Yang, Wen ^{[1
,2
]}

Yao, Ting ^{[1
,2
]}

Wang, Lei ^{[1
,2
,3
]}

机构：

[1] Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China

[2] Nankai Univ, TEDA Inst Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China

[3] Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

来源：

CELL REPORTS | 2022年 / 39卷 / 01期

基金：

中国国家自然科学基金;

关键词：

REGULATES EXPRESSION; BACTERIAL ADHESION; SECRETION SYSTEM; GENOME SEQUENCE; GENE-EXPRESSION; O157-H7; RESISTANCE; TRANSCRIPTION; ATTACHMENT; MULTIDRUG;

D O I：

10.1016/j.celrep.2022.110614

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important extracellular human pathogen. The initial adherence of EHEC to host cells is a major cue for transcriptional induction of the locus of enterocyte effacement (LEE) genes to promote colonization and pathogenesis, but the mechanism through which this adherence is sensed and the LEE is induced remains largely elusive. Here, we report a complete signal transduction pathway for this virulence activation process. In this pathway, the outer-membrane lipoprotein NlpE senses a mechanical cue generated from initial host adherence and activates the BaeSR two-component regulatory system; the response regulator BaeR then directly activates the expression of airA located on O-island-134 and encoding a LEE transcriptional activator. Disruption of this pathway severely attenuates EHEC O157:H7 virulence both in vitro and in vivo. This study provides further insights into the evolution of EHEC pathogenesis and the host-pathogen interaction.

引用

页数：21

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