Evaluation of dynabeads and cytospheres compared with flow cytometry to enumerate CD4+ T cells in HIV-infected Ugandans on antiretroviral therapy
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作者:
Lutwama, Fred
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Acad Alliance AIDS Care & Prevent & Infect Dis In, Kampala, UgandaJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Lutwama, Fred
[2
]
Serwadda, Ronnie
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机构:
Acad Alliance AIDS Care & Prevent & Infect Dis In, Kampala, UgandaJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Serwadda, Ronnie
[2
]
Mayanja-Kizza, Harriet
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机构:
Makerere Univ, Kampala, UgandaJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Mayanja-Kizza, Harriet
[5
]
Shihab, Hasan M.
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Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USAJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Shihab, Hasan M.
[1
]
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Ronald, Allan
[2
,3
]
Kamya, Moses R.
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Acad Alliance AIDS Care & Prevent & Infect Dis In, Kampala, Uganda
Makerere Univ, Kampala, UgandaJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Kamya, Moses R.
[2
,5
]
Thomas, David
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Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USAJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Thomas, David
[1
]
Johnson, Elizabeth
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Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USAJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Johnson, Elizabeth
[1
]
Quinn, Thomas C.
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Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
NIAID, NIH, Bethesda, MD 20892 USAJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Quinn, Thomas C.
[1
,4
]
Moore, Richard D.
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Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USAJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Moore, Richard D.
[1
]
Spacek, Lisa A.
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Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USAJohns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
Spacek, Lisa A.
[1
]
机构:
[1] Johns Hopkins Med Inst, Div Infect Dis, Baltimore, MD 21287 USA
[2] Acad Alliance AIDS Care & Prevent & Infect Dis In, Kampala, Uganda
Background: Laboratory-based monitoring of antiretroviral therapy is essential but adds a significant cost to HIV care. The World Health Organization 2006 guidelines support the use of CD4 lymphocyte count (CD4) to define treatment failure in resource-limited settings. Methods: We compared CD4 obtained on replicate samples from 497 HIV-positive Ugandans (before and during ART) followed for 18 months by 2 manual bead-based assays, Dynabeads (Dynal Biotech), and Cytospheres (Beckman Coulter) with those generated by flow cytometry at the Infectious Diseases Institute in Kampala, Uganda. Results: We tested 1671 samples (123 before ART) with Dynabeads and 1444 samples (91 before ART) with Cytospheres. Mean CD4 was 231 cells/mm(3) (SD, 139) and 239 celIS/MM3 (SD, 140) by Dynabeads and flow cytometry, respectively. Mean CD4 was 186 cells/mm(3) (SD, 10 1) and 242 cells/mm(3) (SD, 13 6) by Cytospheres and flow cytometry, respectively. The mean difference in CD4 count by flow cytometry versus Dynabeads were 8.8 cells/mm(3) (SD, 76.0) and versus Cytospheres were 56.8 cells/mm(3) (SD, 85.8). The limits of agreement were -140.9 to 158.4 cells/mm(3) for Dynabeads and -112.2 to 225.8 cells/mm(3) for Cytospheres. Linear regression analysis showed higher correlation between flow cytometry and Dynabeads (r = 0.85, r(2) = 0.73, slope = 0.85, intercept = 28) compared with the correlation between flow cytometry and Cytospheres (r = 0.78, r(2) = 0.60, slope = 0.58, intercept = 45). Area under the receiver operating characteristics curve to predict CD4 <200 cells/mm(3) was 0.928 for Dynabeads and 0.886 for Cylospheres. Conclusion: Although Dynabeads and Cytospheres both underestimated CD4 lymphocyte count compared with flow cytometry, in resource-limited settings with low daily throughput, manual bead-based assays may provide a less expensive alternative to flow cytometry.
机构:
Univ Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, MalaysiaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Yong, Yean K.
Shankar, Esaki M.
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Univ Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Univ Malaya, Fac Med, Dept Med Microbiol, Trop Infect Dis Res & Educ Ctr TIDREC, Kuala Lumpur, MalaysiaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Shankar, Esaki M.
Solomon, Ajantha
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Univ Melbourne, Peter Doherty Inst Infect & Immun, Level 4,786-798 Elizabeth St, Melbourne, Vic 3010, Australia
Royal Melbourne Hosp, Melbourne, Vic, AustraliaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Solomon, Ajantha
Spelman, Tim
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Univ Melbourne, Peter Doherty Inst Infect & Immun, Level 4,786-798 Elizabeth St, Melbourne, Vic 3010, Australia
Royal Melbourne Hosp, Melbourne, Vic, Australia
Burnet Inst, Ctr Populat Hlth, Melbourne, Vic, Australia
Monash Univ, Sch Publ Hlth & Prevent Med, Melbourne, Vic, AustraliaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Spelman, Tim
Fairley, Christopher K.
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Alfred Hosp, Melbourne Sexual Hlth Ctr, Melbourne, Vic, Australia
Monash Univ, Cent Clin Sch, Melbourne, Vic, AustraliaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Fairley, Christopher K.
Elliott, Julian H.
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Alfred Hosp, Dept Infect Dis, Melbourne, Vic, Australia
Monash Univ, Melbourne, Vic, AustraliaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Elliott, Julian H.
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Hoy, Jennifer
Cameron, Paul U.
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Univ Melbourne, Peter Doherty Inst Infect & Immun, Level 4,786-798 Elizabeth St, Melbourne, Vic 3010, Australia
Royal Melbourne Hosp, Melbourne, Vic, Australia
Alfred Hosp, Dept Infect Dis, Melbourne, Vic, Australia
Monash Univ, Melbourne, Vic, AustraliaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Cameron, Paul U.
Kamarulzaman, Adeeba
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Univ Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
Univ Malaya, Med Ctr, Infect Dis Unit, Kuala Lumpur 50603, MalaysiaUniv Malaya, Fac Med, Ctr Excellence Res AIDS CERiA, Kuala Lumpur, Malaysia
机构:
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, PermInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Shmagel K.V.
Korolevskaya L.B.
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Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, PermInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Korolevskaya L.B.
Saidakova E.V.
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Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, PermInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Saidakova E.V.
Shmagel N.G.
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Perm Regional Center for Protection against AIDS and Infectious Diseases, PermInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Shmagel N.G.
Chereshnev V.A.
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Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences, YekaterinburgInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Chereshnev V.A.
Margolis L.
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National Institute of Child Health and Human Development, Bethesda, MDInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Margolis L.
Anthony D.
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Case Western Reserve University, Cleveland, OHInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
Anthony D.
Lederman M.
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Case Western Reserve University, Cleveland, OHInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm
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Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Bekele, Yonas
Lakshmikanth, Tadepally
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Karolinska Inst, Sci Life Lab, Div Clin Pediat, Dept Womens & Childrens Hlth, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Lakshmikanth, Tadepally
Chen, Yang
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Karolinska Inst, Sci Life Lab, Div Clin Pediat, Dept Womens & Childrens Hlth, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Chen, Yang
Mikes, Jaromir
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Karolinska Inst, Sci Life Lab, Div Clin Pediat, Dept Womens & Childrens Hlth, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Mikes, Jaromir
Nasi, Aikaterini
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Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Nasi, Aikaterini
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Petkov, Stefan
Hejdeman, Bo
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Karolinska Inst, Dept Clin Sci & Educ, Sodersjukhuset, Stockholm, Sweden
Venhalsan, Unit Infect Dis, Sodersjukhuset, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Hejdeman, Bo
Brodin, Petter
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Karolinska Inst, Sci Life Lab, Div Clin Pediat, Dept Womens & Childrens Hlth, Stockholm, Sweden
Karolinska Univ Hosp, Dept Newborn Med, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
Brodin, Petter
Chiodi, Francesca
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Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, SwedenKarolinska Inst, Dept Microbiol Tumor & Cell Biol, Biomedicum, Stockholm, Sweden
机构:
Guangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R ChinaGuangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R China
Su, Qi-jian
Li, Yi-zhong
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Guangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R ChinaGuangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R China
Li, Yi-zhong
Liang, Fei-li
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Peoples Hosp Hengxian Cty, Div Infect Dis, Nanning, Guangxi, Peoples R ChinaGuangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R China
Liang, Fei-li
Xiao, Jian
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Guangxi Univ Chinese Med, Fac Preclin Med, Nanning 530011, Guangxi, Peoples R ChinaGuangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R China
Xiao, Jian
Deng, Xin
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Guangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R ChinaGuangxi Univ Chinese Med, Ruikang Hosp, Ctr AIDS Res, Nanning 530011, Guangxi, Peoples R China
机构:
British Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
US Ctr Dis Control & Prevent, Global AIDS Program, Entebbe, Uganda
Univ British Columbia, Fac Med, Dept Med, Vancouver, BC, CanadaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Moore, David M.
Awor, Anna
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US Ctr Dis Control & Prevent, Global AIDS Program, Entebbe, UgandaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Awor, Anna
Downing, Robert
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US Ctr Dis Control & Prevent, Global AIDS Program, Entebbe, UgandaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Downing, Robert
Kaplan, Jonathan
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US Ctr Dis Control & Prevent, Global AIDS Program, Atlanta, GA USABritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Kaplan, Jonathan
Montaner, Julio S. G.
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British Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Univ British Columbia, Fac Med, Dept Med, Vancouver, BC, CanadaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Montaner, Julio S. G.
Hancock, John
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US Ctr Dis Control & Prevent, Global AIDS Program, Entebbe, UgandaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Hancock, John
Were, Willy
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US Ctr Dis Control & Prevent, Global AIDS Program, Entebbe, UgandaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
Were, Willy
Mermin, Jonathan
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US Ctr Dis Control & Prevent, Global AIDS Program, Entebbe, UgandaBritish Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6T 1Y6, Canada
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Univ Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Ho, Jennifer E.
Deeks, Steven G.
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Univ Calif San Francisco, Dept Med, Posit Hlth Program, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Deeks, Steven G.
Hecht, Frederick M.
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Univ Calif San Francisco, Dept Med, Posit Hlth Program, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Hecht, Frederick M.
Xie, Yu
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Univ Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Xie, Yu
Schnell, Amanda
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Univ Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Schnell, Amanda
Martin, Jeffrey N.
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Univ Calif San Francisco, Dept Med, Posit Hlth Program, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Martin, Jeffrey N.
Ganz, Peter
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Univ Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
Ganz, Peter
Hsue, Priscilla Y.
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Univ Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USAUniv Calif San Francisco, Div Cardiol, Dept Med, San Francisco Gen Hosp, San Francisco, CA 94110 USA
机构:
Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, CBAR, Boston, MA 02115 USAEunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
Patel, Kunjal
Van Dyke, Russell B.
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Tulane Univ Hlth Sci Ctr, New Orleans, LA USAEunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
Van Dyke, Russell B.
Hazra, Rohan
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机构:Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
Hazra, Rohan
Burchett, Sandra K.
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Childrens Hosp, Boston, MA 02115 USA
Harvard Univ, Sch Med, Boston, MA USAEunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
Burchett, Sandra K.
Spector, Stephen A.
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Univ Calif San Diego, La Jolla, CA 92093 USA
Rady Childrens Hosp San Diego, San Diego, CA USAEunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
机构:Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
Read, Jennifer S.
Wiznia, Andrew
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Albert Einstein Coll Med, Jacobi Med Ctr, New York, NY USAEunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA
Wiznia, Andrew
Seage, George R., III
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Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA
Harvard Univ, Sch Publ Hlth, CBAR, Boston, MA 02115 USAEunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS PAMA Branch, Ctr Res Mothers & Children, NIH, Bethesda, MD 20892 USA