Pitfalls Associated With the Use of Molecular Diagnostic Panels in the Diagnosis of Cryptococcal Meningitis

被引：31

作者：

O'Halloran, Jane A. ^{[1
]}

Franklin, Alexander ^{[2
]}

Lainhart, William ^{[3
]}

Burnham, Carey-Ann ^{[3
]}

Powderly, William ^{[1
]}

Dubberke, Erik ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Div Infect Dis, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

来源：

OPEN FORUM INFECTIOUS DISEASES | 2017年 / 4卷 / 04期

关键词：

cryptococcal meningitis; encephalitis; meningitis; molecular diagnostic techniques; polymerase chain reaction; MENINGITIS/ENCEPHALITIS PANEL; NEOFORMANS; IMPACT;

D O I：

10.1093/ofid/ofx242

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We report the case of a kidney transplantation patient on chronic immunosuppressive therapy presenting with subacute meningitis. The final diagnosis of cryptococcal meningitis was delayed due to 2 false-negative cryptococcal results on a molecular diagnostic panel. Caution with such platforms in suspected cryptococcal meningitis is needed.

引用

页数：3

共 10 条

[1] Cryptococcosis-the impact of delay to diagnosis [J].

Aye, C. ;

Henderson, A. ;

Yu, H. ;

Norton, R. .

CLINICAL MICROBIOLOGY AND INFECTION, 2016, 22 (07) :632-635

[2] Predictors of Mortality and Differences in Clinical Features among Patients with Cryptococcosis According to Immune Status [J].

Brizendine, Kyle D. ;

Baddley, John W. ;

Pappas, Peter G. .

PLOS ONE, 2013, 8 (03)

[3] Synergistic antifungal activities of bafilomycin A1, fluconazole, and the pneumocandin MK-0991/caspofungin acetate (L-743,873) with calcineurin inhibitors FK506 and L-685,818 against Cryptococcus neoformans [J].

Del Poeta, M ;

Cruz, MC ;

Cardenas, ME ;

Perfect, JR ;

Heitman, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (03) :739-746

[4] Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens [J].

Hanson, K. E. ;

Slechta, E. S. ;

Killpack, J. A. ;

Heyrend, C. ;

Lunt, T. ;

Daly, J. A. ;

Hemmert, A. C. ;

Blaschke, A. J. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (03) :785-787

[5] The First Fully Automated Molecular Diagnostic Panel for Meningitis and Encephalitis: How Well Does It Perform, and When Should It Be Used? [J].

Hanson, Kimberly E. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (09) :2222-2224

[6] Evolution of Cryptococcal Antigen Testing: What Is New? [J].

Nalintya E. ;

Kiggundu R. ;

Meya D. .

Current Fungal Infection Reports, 2016, 10 (2) :62-67

[7]

Rajasingham R, 2017, LANCET INFECT DIS, V17, P873, DOI [10.1016/S1473-3099(17)30243-8, 10.1016/s1473-3099(17)30243-8]

[8] Diagnostic performance of a multiplex PCR assay for meningitis in an HIV-infected population in Uganda [J].

Rhein, Joshua ;

Bahr, Nathan C. ;

Hemmert, Andrew C. ;

Cloud, Joann L. ;

Bellamkonda, Satya ;

Oswald, Cody ;

Lo, Eric ;

Nabeta, Henry ;

Kiggundu, Reuben ;

Akampurira, Andrew ;

Musubire, Abdu ;

Williams, Darlisha A. ;

Meya, David B. ;

Boulware, David R. .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2016, 84 (03) :268-273

[9] Cryptococcus neoformans in organ transplant recipients:: Impact of calcineurin-inhibitor agents on mortality [J].

Singh, Nina ;

Alexander, Barbara D. ;

Lortholary, Olivier ;

Dromer, Francoise ;

Gupta, Krishan L. ;

John, George T. ;

del Busto, Ramon ;

Klintmalm, Goran B. ;

Somani, Jyoti ;

Lyon, G. Marshall ;

Pursell, Kenneth ;

Stosor, Valentina ;

Munoz, Patricia ;

Limaye, Ajit P. ;

Kalil, Andre C. ;

Pruett, Timothy L. ;

Garcia-Diaz, Julia ;

Humar, Atul ;

Houston, Sally ;

House, Andrew A. ;

Wray, Dannah ;

Orloff, Susan ;

Dowdy, Lorraine A. ;

Fisher, Robert A. ;

Heitman, Joseph ;

Wagener, Marilyn M. ;

Husain, Shahid .

JOURNAL OF INFECTIOUS DISEASES, 2007, 195 (05) :756-764

[10] Enhancing pathogen identification in patients with meningitis and a negative Gram stain using the BioFire FilmArray® Meningitis/Encephalitis panel [J].

Wootton, Susan H. ;

Aguilera, Elizabeth ;

Salazar, Lucrecia ;

Hemmert, Andrew C. ;

Hasbun, Rodrigo .

ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS, 2016, 15

← 1 →