Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

被引：31
|
作者：
Vlahos, Katerina [1 ]
Sourris, Koula [1 ]
Mayberry, Robyn [1 ]
McDonald, Penelope [1 ]
Bruveris, Freya F. [1 ,2 ]
Schiesser, Jacqueline V. [1 ,2 ]
Bozaoglu, Kiymet [1 ,2 ,3 ]
Lockhart, Paul J. [1 ,2 ,3 ]
Stanley, Edouard G. [1 ,2 ,4 ]
Elefanty, Andrew G. [1 ,2 ,4 ]
机构：
[1] Royal Childrens Hosp, Murdoch Childrens Res Inst, Melbourne, Vic 3052, Australia
[2] Univ Melbourne, Dept Paediat, Parkville, Vic 3052, Australia
[3] Murdoch Childrens Res Inst, Bruce Lefroy Ctr Genet Hlth Res, Parkville, Vic 3052, Australia
[4] Monash Univ, Dept Anat & Dev Biol, Clayton, Vic 3800, Australia
来源：
STEM CELL RESEARCH | 2019年 / 34卷
基金：
英国医学研究理事会;
关键词：
PLURIPOTENT STEM-CELLS; DIFFERENTIATION; MESODERM;
D O I：
10.1016/j.scr.2018.101380
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.
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页数：5
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