Colonization of Tomato Seedlings by Bioluminescent Clavibacter michiganensis subsp michiganensis Under Different Humidity Regimes

被引:25
|
作者
Xu, Xiulan [1 ]
Rajashekara, Gireesh [2 ]
Paul, Pierce A. [1 ]
Miller, Sally A. [1 ]
机构
[1] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA
[2] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food & Anim Hlth Res Program, Wooster, OH 44691 USA
关键词
PSEUDOMONAS-SYRINGAE; BACTERIAL CANKER; TWITCHING MOTILITY; HIGH-THROUGHPUT; IN-PLANTA; INFECTION; GROWTH; LEAVES; HOST;
D O I
10.1094/PHYTO-03-11-0090
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Tomato bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis, is transmitted by infected or infested seed and mechanically from plant to plant. Wounds occurring during seedling production and crop maintenance facilitate the dissemination of the pathogen. However, the effects of environmental factors on C. michiganensis subsp. michiganensis translocation and growth as an endophyte have not been fully elucidated. A virulent, stable, constitutively bioluminescent C. michiganensis subsp. michiganensis strain BL-Cmm 17 coupled with an in vivo imaging system allowed visualization of the C. michiganensis subsp. michiganensis colonization process in tomato seedlings in real time. The dynamics of bacterial infection in seedlings through wounds were compared under low (45%) and high (83%) relative humidity. Bacteria multiplied rapidly in cotyledon petioles remaining after clip inoculation and moved in the stem toward both root and shoot. Luminescent signals were also observed in tomato seedling roots over time, and root development was reduced in inoculated plants maintained under both humidity regimes. Wilting was more severe in seedlings under high-humidity regimes. A strong positive correlation between light intensity and bacterial population in planta suggests that bioluminescent C. michiganensis subsp. michiganensis strains will be useful in evaluating the efficacy of bactericides and host resistance.
引用
收藏
页码:177 / 184
页数:8
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