High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels

被引：7

作者：

Bian, Yingnan ^{[1
]}

Zhou, Wei ^{[1
]}

Zhao, Yingchun ^{[1
]}

Li, Xiaoping ^{[1
]}

Geng, Wei ^{[1
]}

Hao, Ruixin ^{[1
]}

Yang, Qing ^{[1
]}

Huang, Weida ^{[1
,2
]}

机构：

[1] Fudan Univ, Sch Life Sci, Dept Biochem, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Adv Res Inst, Lab Synthet Biol, Ctr Nanomed, Shanghai, Peoples R China

来源：

PLOS ONE | 2011年 / 6卷 / 10期

关键词：

GENE-EXPRESSION; HISTONE DEACETYLASE; PROMOTER ACTIVITY; SIGNAL TRANSDUCER; INTERFERING RNAS; MESSENGER-RNA; DNA-BINDING; IN-VIVO; C-JUN; EPITHELIAL-CELLS;

D O I：

10.1371/journal.pone.0026466

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.

引用

页数：15

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