High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels

被引：7

作者：

Bian, Yingnan ^{[1
]}

Zhou, Wei ^{[1
]}

Zhao, Yingchun ^{[1
]}

Li, Xiaoping ^{[1
]}

Geng, Wei ^{[1
]}

Hao, Ruixin ^{[1
]}

Yang, Qing ^{[1
]}

Huang, Weida ^{[1
,2
]}

机构：

[1] Fudan Univ, Sch Life Sci, Dept Biochem, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Adv Res Inst, Lab Synthet Biol, Ctr Nanomed, Shanghai, Peoples R China

来源：

PLOS ONE | 2011年 / 6卷 / 10期

关键词：

GENE-EXPRESSION; HISTONE DEACETYLASE; PROMOTER ACTIVITY; SIGNAL TRANSDUCER; INTERFERING RNAS; MESSENGER-RNA; DNA-BINDING; IN-VIVO; C-JUN; EPITHELIAL-CELLS;

D O I：

10.1371/journal.pone.0026466

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.

引用

页数：15

共 94 条

[21] Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7
Fischer, Sylvia E. J.
Butler, Maurice D.
Pan, Qi
Ruvkun, Gary
[J]. NATURE, 2008, 455 (7212) : 491 - U23
[22] Connexin32 as a tumor suppressor gene in a metastatic renal cell carcinoma cell line
Fujimoto, E
Sato, H
Shirai, S
Nagashima, Y
Fukumoto, K
Hagiwara, H
Negishi, E
Ueno, K
Omori, Y
Yamasaki, H
Hagiwara, K
Yano, T
[J]. ONCOGENE, 2005, 24 (22) : 3684 - 3690
[23] Inhibition of N-cadherin and β-catenin function reduces axon-induced Schwann cell proliferation
Gess, Burkhard
Halfter, Hartmut
Kleffner, Ilka
Monje, Paula
Athauda, Gagani
Wood, Patrick M.
Young, Peter
Wanner, Ina B.
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2008, 86 (04) : 797 - 812
[24] Sp1 is required for the early response of alpha 2(I) collagen to transforming growth factor-beta 1
Greenwel, P
Inagaki, Y
Hu, W
Walsh, M
Ramirez, F
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (32) : 19738 - 19745
[25] High doses of siRNAs induce eri-1 and adar-1 gene expression and reduce the efficiency of RNA interference in the mouse
Hong, J
Qian, ZK
Shen, SY
Min, TS
Tan, C
Xu, JF
Zhao, YC
Huang, WD
[J]. BIOCHEMICAL JOURNAL, 2005, 390 : 675 - 679
[26] esiRNA to eri-1 and adar-1 genes improving high doses of c-myc-directed esiRNA effect on mouse melanoma growth inhibition
Hong, Jie
Zhao, Yinchun
Li, Zhengwu
Huang, Weida
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 361 (02) : 373 - 378
[27] Blocking c-myc and stat3 by E. coli expressed and enzyme digested siRNA in mouse melanoma
Hong, Jie
Zhao, Yingchun
Huang, Weida
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 348 (02) : 600 - 605
[28] Trichostatin a induces transforming growth factor ß type II receptor promoter activity and acetylation of Sp1 by recruitment of PCAF/p300 to a Sp1•NF-Y complex
Huang, WQ
Zhao, SJ
Ammanamanchi, S
Brattain, M
Venkatasubbarao, K
Freeman, JW
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (11) : 10047 - 10054
[29] Sp1 deacetylation induced by phorbol ester recruits p300 to activate 12(S)-lipoxygenase gene transcription
Hung, JJ
Wang, YT
Chang, WC
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (05) : 1770 - 1785
[30] Expression profiling reveals off-target gene regulation by RNAi
Jackson, AL
Bartz, SR
Schelter, J
Kobayashi, SV
Burchard, J
Mao, M
Li, B
Cavet, G
Linsley, PS
[J]. NATURE BIOTECHNOLOGY, 2003, 21 (06) : 635 - 637

← 1 2 3 4 5 6 7 8 9 10 →