High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels

被引：7

作者：

Bian, Yingnan ^{[1
]}

Zhou, Wei ^{[1
]}

Zhao, Yingchun ^{[1
]}

Li, Xiaoping ^{[1
]}

Geng, Wei ^{[1
]}

Hao, Ruixin ^{[1
]}

Yang, Qing ^{[1
]}

Huang, Weida ^{[1
,2
]}

机构：

[1] Fudan Univ, Sch Life Sci, Dept Biochem, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Adv Res Inst, Lab Synthet Biol, Ctr Nanomed, Shanghai, Peoples R China

来源：

PLOS ONE | 2011年 / 6卷 / 10期

关键词：

GENE-EXPRESSION; HISTONE DEACETYLASE; PROMOTER ACTIVITY; SIGNAL TRANSDUCER; INTERFERING RNAS; MESSENGER-RNA; DNA-BINDING; IN-VIVO; C-JUN; EPITHELIAL-CELLS;

D O I：

10.1371/journal.pone.0026466

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.

引用

页数：15

共 94 条

[1] Signal Transducer and Activator of Transcription-3, Inflammation, and Cancer How Intimate Is the Relationship? [J].

Aggarwal, Bharat B. ;

Kunnumakkara, Ajaikurnar B. ;

Harikumar, Kuzhuvelil B. ;

Gupta, Shan R. ;

Tharakan, Sheeja T. ;

Koca, Cemile ;

Dey, Sanjit ;

Sung, Bokyung .

NATURAL COMPOUNDS AND THEIR ROLE IN APOPTOTIC CELL SIGNALING PATHWAYS, 2009, 1171 :59-76

[2] MRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes [J].

Behm-Ansmant, Isabelle ;

Rehwinkel, Jan ;

Doerks, Tobias ;

Stark, Alexander ;

Bork, Peer ;

Izaurralde, Elisa .

GENES & DEVELOPMENT, 2006, 20 (14) :1885-1898

[3] SECRETED PLACENTAL ALKALINE-PHOSPHATASE - A POWERFUL NEW QUANTITATIVE INDICATOR OF GENE-EXPRESSION IN EUKARYOTIC CELLS [J].

BERGER, J ;

HAUBER, J ;

HAUBER, R ;

GEIGER, R ;

CULLEN, BR .

GENE, 1988, 66 (01) :1-10

[4] Growth/cell cycle regulation of Sp1 phosphorylation [J].

Black, AR ;

Jensen, D ;

Lin, SY ;

Azizkhan, JC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (03) :1207-1215

[5] Regulation of the activity of Sp1-related transcription factors [J].

Bouwman, P ;

Philipsen, S .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2002, 195 (1-2) :27-38

[6]

Burgyan Jozsef, 2008, V451, P69, DOI 10.1007/978-1-59745-102-4_5

[7] STAT3 beta, a splice variant of transcription factor STAT3, is a dominant negative regulator of transcription [J].

Caldenhoven, E ;

vanDijk, TB ;

Solari, R ;

Armstrong, J ;

Raaijmakers, JAM ;

Lammers, JWJ ;

Koenderman, L ;

deGroot, RP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (22) :13221-13227

[8] Specific interaction of heterogeneous nuclear ribonucleoprotein A1 with the-219T allelic form modulates APOE promoter activity [J].

Campillos, M ;

Lamas, JRN ;

García, MA ;

Bullido, MJ ;

Valdivieso, F ;

Vázquez, J .

NUCLEIC ACIDS RESEARCH, 2003, 31 (12) :3063-3070

[9] Interleukin-18-induced human coronary artery smooth muscle cell migration is dependent on NF-κB- and AP-1-mediated matrix metalloproteinase-9 expression and is inhibited by atorvastatin [J].

Chandrasekar, Bysani ;

Mummidi, Srinivas ;

Mahimainathan, Lenin ;

Patel, Devang N. ;

Bailey, Steven R. ;

Imam, Syed Z. ;

Greene, Warner C. ;

Valente, Anthony J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (22) :15099-15109

[10] Functional interaction between c-Jun and promoter factor Sp1 in epidermal growth factor-induced gene expression of human 12(S)-lipoxygenase [J].

Chen, BK ;

Chang, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (19) :10406-10411

← 1 2 3 4 5 6 7 8 9 10 →