High-Dose siRNAs Upregulate Mouse Eri-1 at both Transcription and Posttranscription Levels

被引：7
作者：
Bian, Yingnan [1 ]
Zhou, Wei [1 ]
Zhao, Yingchun [1 ]
Li, Xiaoping [1 ]
Geng, Wei [1 ]
Hao, Ruixin [1 ]
Yang, Qing [1 ]
Huang, Weida [1 ,2 ]
机构：
[1] Fudan Univ, Sch Life Sci, Dept Biochem, Shanghai 200433, Peoples R China
[2] Chinese Acad Sci, Shanghai Adv Res Inst, Lab Synthet Biol, Ctr Nanomed, Shanghai, Peoples R China
来源：
PLOS ONE | 2011年 / 6卷 / 10期
关键词：
GENE-EXPRESSION; HISTONE DEACETYLASE; PROMOTER ACTIVITY; SIGNAL TRANSDUCER; INTERFERING RNAS; MESSENGER-RNA; DNA-BINDING; IN-VIVO; C-JUN; EPITHELIAL-CELLS;
D O I：
10.1371/journal.pone.0026466
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.
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页数：15
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