Expression of autophagy-related genes in the anterior silk gland of the silkworm (Bombyx mori) during metamorphosis

被引：0

作者：

Li, Qingrong ^{[1
]}

Deng, Xiaojuan ^{[2
]}

Huang, Zhijun ^{[2
]}

Zheng, Sichun ^{[1
]}

Tettamanti, Gianluca ^{[3
]}

Cao, Yang ^{[2
]}

Feng, Qili ^{[1
]}

机构：

[1] S China Normal Univ, Guangdong Prov Key Lab Biotechnol Plant Dev, Sch Life Sci, Guangzhou 510631, Guangdong, Peoples R China

[2] S China Agr Univ, Lab Insect Mol Biol & Biotechnol, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

[3] Univ Insubria, Dept Biotechnol & Life Sci, I-21100 Varese, Italy

来源：

CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE | 2011年 / 89卷 / 11期

关键词：

PROGRAMMED CELL-DEATH; APOPTOTIC FEATURES; CONJUGATION SYSTEM; PREPUPAL INSTAR; SELF-DIGESTION; MANDUCA-SEXTA; YEAST; LC3; ECDYSONE; PATHWAY;

D O I：

10.1139/Z11-075

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Programmed cell death (PCD) is a genetically regulated process of cell elimination and is evolutionarily conserved in eukaryotes. Degeneration of larval tissues during metamorphosis in insects is a result of PCD triggered by ecdysteroids and autophagic process has been shown to be involved in the degeneration of silk gland of the silkworm (Bombyx mori L., 1758). However, experimental evidence for the expression of autophagy marker genes remains insufficient. In this study, expression of the autophagy-related genes BmAtg5, BmAtg6, BmAtg8, and BmAtg12 was determined in the anterior silk glands of larvae and pupae during larval to pupal transformation by using reverse-transcription PCR, quantitative real-time PCR, and Western blot analyses. The results indicated that BmAtg5, BmAtg6, BmAtg8, and BmAtg12 had the highest expression levels on the 3rd day of the wandering stage or at the prepupal stage, and the time frame of the expression of these genes was coincident with the morphological characteristics of autophagy in the silk gland during the larval to pupal transformation. This study demonstrated that the autophagy-related genes are involved in the degeneration of the anterior silk gland of B. mori.

引用

页码：1019 / 1026

页数：8

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