Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

被引:1
作者
de Vor, Lisanne [1 ]
van Dijk, Bruce [2 ]
van Kessel, Kok [1 ]
Kavanaugh, Jeffrey S. [3 ]
de Haas, Carla [1 ]
Aerts, Piet C. [1 ]
Viveen, Marco C. [1 ]
Boel, Edwin C. [1 ]
Fluit, Ad C. [1 ]
Kwiecinski, Jakub M. [3 ]
Krijger, Gerard C. [4 ]
Ramakers, Ruud M. [5 ,6 ,7 ]
Beekman, Freek J. [5 ,6 ,7 ]
Dadachova, Ekaterina [8 ]
Lam, Marnix G. E. H. [4 ]
Vogely, H. Charles [2 ]
van der Wal, Bart C. H. [2 ]
van Strijp, Jos A. G. [1 ]
Horswill, Alexander R. [3 ,9 ]
Weinans, Harrie [2 ,10 ]
Rooijakkers, Suzan H. M. [1 ]
机构
[1] Univ Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands
[2] Univ Med Ctr Utrecht, Dept Orthoped, Utrecht, Netherlands
[3] Univ Colorado, Dept Immunol & Microbiol, Sch Med, Aurora, CO USA
[4] Univ Med Ctr Utrecht, Dept Radiol & Nucl Med, Utrecht, Netherlands
[5] MILabs BV, Utrecht, Netherlands
[6] Univ Med Ctr, Brain Ctr Rudolf Magnus, Dept Translat Neurosci, Utrecht, Netherlands
[7] Delft Univ Technol, Dept Radiat Sci & Technol, Delft, Netherlands
[8] Univ Saskatchewan, Coll Pharm & Nutr, Saskatoon, SK, Canada
[9] Eastern Colorado Hlth Care Syst, Dept Vet Affairs, Denver, CO USA
[10] Delft Univ Technol, Dept Biomech Engn, Delft, Netherlands
基金
美国国家卫生研究院;
关键词
Staphylococcus aureus; biofilm; monoclonal antibodies; Other; POLYSACCHARIDE INTERCELLULAR ADHESIN; PROTEIN CLUMPING FACTOR; CRYSTAL-STRUCTURE; IMMUNOGLOBULIN-G; GENE-EXPRESSION; INFECTION; USA300; SPECT; SUSCEPTIBILITY; ACCUMULATION;
D O I
10.7554/eLife.67301; 10.7554/eLife.67301.sa0; 10.7554/eLife.67301.sa1; 10.7554/eLife.67301.sa2
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.
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页数:25
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