First report of bovine herpesvirus 1 isolation from bull semen samples in China

被引:19
作者
Zhu, L. [1 ,2 ]
Yu, Y. [1 ,2 ]
Jiang, X. [1 ,2 ]
Yuan, W. [3 ]
Zhu, G. [1 ,2 ]
机构
[1] Yangzhou Univ, Coll Vet Med, 48 Wenhui East Rd, Yangzhou 225009, Jiangsu, Peoples R China
[2] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, 48 Wenhui East Rd, Yangzhou 225009, Jiangsu, Peoples R China
[3] Chinese Acad Agr Sci, Inst Anim Sci, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China
基金
美国国家科学基金会;
关键词
BoHV-1; virus isolation; VP16; CPE; ARTIFICIAL-INSEMINATION; TYPE-1; BHV-1; INFECTION; VIRUS; COFACTOR; BIOLOGY;
D O I
10.4149/av_2017_411
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bovine herpesvirus 1 (BoHV-1) infection causes substantial economic losses to the cattle industry worldwide. So far, the isolation of BoHV-1 field virus has not been reported in China. Here, for the first time we report that two isolates of BoHV-1 designated as NJ16-1 and NJ16-2 were obtained from semen samples from breeding bulls in China. Typical cytopathic effect in MDBK cells, detection of viral protein VP16 in western blot analysis, PCR detection of BoHV-1 gB gene proved BoHV-1 infection and subsequent nucleotide sequence analysis showed a 99% identity with BoHV-1 Cooper strain. These results suggest that these isolated viruses are BoHV-1.
引用
收藏
页码:483 / 486
页数:4
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